University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Agricultural -> grain -> sorghum

Citations 1

"Amino-acid Analysis Of Feedstuff Hydrolysates By Pre-column Derivatization With Phenyl Isothiocyanate And Reversed-phase High Performance Liquid Chromatography"
Cereal Chem. 1987 Volume 64, Issue 4 Pages 226-229
R. G. Elkin and A. M. Wasynczuk

Abstract: Maize, peanut meal, two sorghum varieties and a maize - soya-bean meal-based complete mixed feed were either untreated or oxidized with performic acid before hydrolysis with HCl. Amino-acids in the hydrolysate were derivatized with phenyl isothiocyanate and the resulting phenylthiocarbamoyl derivatives were analyzed by HPLC on a column (15 cm x 3.9 mm) of Pico-Tag operated at 33°C. Elution was effected with a gradient from 90 to 0 to 90% of 0.14 M Na acetate containing 0.7 mL L-1 of triethanolamine in aqueous 60% acetonitrile and detection was at 254 nm. Amino-acids were also determined in hydrolysates by cation-exchange chromatography (J. Assoc. Off. Anal. Chem., 1985, 68, 1028) and post-column derivatization with ninhydrin. Amino-acid values by HPLC generally agreed with those by cation-exchange chromatography. Complete separation of 17 hydrolysate amino-acids plus methionine and cysteic acid was achieved in 25 min by HPLC and, thus, use of modular HPLC equipment provides a viable alternative to the use of amino-acid analyzers.
Amino Acids HPLC Spectrophotometry Heated reaction Post-column derivatization Pre-column derivatization