University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Malt Flour

Classification: Agricultural -> grain -> malt -> flour

Citations 3

"Size Exclusion Chromatographic Determination Of β-glucan With Post-column Reaction Detection"
J. Chromatogr. A 1993 Volume 632, Issue 1-2 Pages 105-110
T. Suortti

Abstract: An extract of barley or malt was analyzed at 65°C on columns of µHydrogel 250 and 2000 in series, with aqueous 50 mM NaOH as mobile phase. A solution of calcofluor in 50 mM NaOH was mixed with the eluate, and the product of the specific reaction of β-glucan with the dye was measured fluorimetrically at 445 nm (excitation at 415 nm) or by UV absorbance. Results showed good correlation with those obtained by flow injection analysis with calcofluor as reagent. Using the specific interaction of β-glucan with calcofluor dye as a detection method for size-exclusion chromatographic analysis, very simple direct extractions can be used for sample preparation. Either fluorescence or UV detection is employed. The calibration graphs are linear between 200 and 20 mg/l, although much more sensitive analyzes are possible. The response remained independent of relative molecular mass in the range studied (1.7 x 10(6)-1.85 x 10(5)). Results for barley and malt show good correlation with those obtained by flow injection analysis using calcofluor.
Chromatography Spectrophotometry Post-column derivatization

"Direct Assay Of S-methylmethionine Using High Performance Liquid Chromatography And Fluorescence Techniques"
J. Am. Soc. Brew. Chem. 1986 Volume 44, Issue 1 Pages 1-6
Jean Pierre Dufour

Abstract: Malt flour was extracted with 0.1% trifluoroacetic acid(I) at 0°C. To the extract or to a sample of wort or decarbonated beer were added 1 mM L-2-amino-3-guanidinopropionic acid (internal standard) and 0.1% I - methanol (7:3). The solution was cleaned up on a Sep-Pak C18 cartridge. The eluate was analyzed by HPLC on a Waters amino-acid column with post-column derivatization of S-methylmethionine with phthalaldehyde and fluorimetric detection at 460 nm (excitation at 355 nm). The limit of determination was 0.2 µg g-1 of S-methylmethionine in dry malt.
S-Methylmethionine HPLC Fluorescence Post-column derivatization

"Determination Of Total (1->3),(1->4)-β-glucanase Activity By A Calcofluor Flow Injection Analysis Method-D-glucan In Barley And Malt Flour Samples"
J. Cereal Sci. 1996 Volume 23, Issue 3 Pages 293-296
Manzanares P. and Sendra J. M.

Abstract: Flour (0.2 g for barley, 1 g for malt) was mixed with 20 mL 0.05 M HNO3 and heated on a boiling water bath for 20 min. After cooling, the mixture was centrifuged, the supernatant was filtered and the residue was re-extracted with 5 mL 0.05 M HNO3. Total (1->3),(1->4)-β-D-glucan was determined by the fluorimetric Calcofluor-FIA method (J. Inst. Brewing, 1991, 97, 101). Results were compared to those obtained by the European Brewery Convention method (Jorgensen and Aastrup, Carlsberg Res. Communications, 1988, 53, 287) which involves extraction with hot water and H2SO4. Recoveries were 8-24% and 17-27% higher for barley and malt, respectively, using the HNO3 extraction. The RSD was 1.02%.
(1->3),(1->4)-β-d-Glucan Fluorescence Standard method Method comparison