University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Chicken Feed

Classification: Agricultural -> feed -> chicken

Citations 3

"Determination Of Narasin In Raw Material, Premix, And Animal Feeds By Liquid Chromatography And Correlation To Microbiological Assay"
J. AOAC Int. 1994 Volume 77, Issue 4 Pages 821-828
Rodewald, J.M.;Moran, J.W.;Donoho, A.L.;Coleman, M.R.

Abstract: Ground sample was extracted with methanol/H2O (9:1). Analysis was by LC on a column (25 cm x 4.6 mm i.d.) of Partisil 5 ODS-3 (particle size not given), with methanol/H2O/acetic acid (940:60:1) as mobile phase (0.7 ml/min) and post-column derivatization at 98°C in a stainless-steel reaction chamber with vanillin reagent [methanol/H2SO4/vanillin (95:2:3); protected from UV light] introduced via a 90°C mixing tee. Detection was at 520 nm. A diagram of the experimental set-up is given. The LC responses of narasin and I-like factors were determined and correlated to the microbiological response of each factor determined using Streptococcus faecium (details given). Calibration graphs were ranged from 2.5-25 and 25-100 ppm of narasin in cattle and poultry feeds, respectively. The quantitation limit was 5 ppm of narasin. Recoveries were 93.9%-98.3%. The method can separate narasin factors A and D + I from monensin factors A and B, and can also separate narasin from monensin and salinomycin. Lasalocid could not be detected by this system as it is unreactive with vanillin.
Narasin LC

"Liquid Chromatographic Determination Of Amprolium In Poultry Feed And Pre-mixes Using Post-column Chemistry With Fluorimetric Detection"
J. AOAC Int. 1987 Volume 70, Issue 5 Pages 920-922
Vanderslice JT, Huang MH.

Abstract: Feed mixture was mixed with the internal standard (thiamine monophosphate), 5% sulfosalicylic acid and hexane. The aqueous phase was analyzed as described previously (J. Agric. Food Chem., 1980, 28, 1145 and J. Micronutr. Anal., 1986, 2, 189). Pre-mix was mixed with the internal standard (pyrithiamine), water and hexane. After centrifugation, the aqueous phase was subjected to HPLC (loc. cit.) with post-column derivatization with K3Fe(CN)6 in NaOH and fluorimetric detection at 432 nm (excitation at 339 nm). Limits of detection were in the pmol range.
Amprolium HPLC Fluorescence Post-column derivatization

"High-speed Liquid Chromatographic Determination Of Monensin, Narasin And Salinomycin In Feeds, Using Post-column Derivatization"
J. AOAC Int. 1988 Volume 71, Issue 3 Pages 480-484
Lapointe MR, Cohen H

Abstract: Ground sample (20 g) was stirred for 2 h with 200 mL of hexane - ethyl acetate (9:1). After settling, a portion of supernatant solution equivalent to 1 g was evaporated to dryness at 40°C. The residue was dissolved in methanol and a portion of the solution, after filtration, was analyzed by HPLC on a column (6 cm x 0.46 mm) of C18 (3 µm), with methanol - aqueous 5% acetic acid (9:1) as mobile phase (0.5 mL min-1), post-column reaction at 95°C for 1 min with 4% vanillin solution in methanolic 2% H2SO4 (flow rate 1.0 mL min-1), and 520-nm detection. The ranges of rectilinear response were 10 to 100 ng for monensin(I) and 50 to 300 ng for salinomycin(II) and narasin(III). Detection limits were 0.5 ppm for I and 1 ppm for II and III. The average recovery of all three ionophores added to a hatching ration mix was 98.1% with a coefficient of variation of 3.6%. The results of analyzes of medicated poultry feeds and a beef feed lot supplement for I and II were comparable to those obtained by bioassay methods.
Monensin Narasin Salinomycin HPLC Spectrophotometry Heated reaction Method comparison Post-column derivatization