University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Whole Egg

Classification: Agricultural -> dairy -> egg -> whole

Citations 4

"Monitoring Of Veterinary Drug Residues By A Combination Of Continuous-flow Techniques And Column-switching High Performance Liquid Chromatography. 1. Sulfonamides In Egg, Meat And Milk Using Post-column Derivatization With Dimethylaminobenzaldehyde"
J. Chromatogr. A 1988 Volume 435, Issue 1 Pages 97-112
M. M. L. Aerts and W. M. J. Beek, U. A. Th. Brinkman

Abstract: Aqueous NaCl - NaN3 extracts or dilutions of egg, meat or milk were dialysed online vs. water or dilute buffer solution in a flat cellulose membrane. The diffusate was passed through an XAD-4 column (6 cm x 4.6 mm) and the drugs were back-flushed on to a LiChrosorb RP-8, Cp TM-Spher C18 or µBondapak C18 column for HPLC with detection at 280 nm, or at 450 nm following post-column derivatization with 4-dimethylaminobenzaldehyde. The choice of stationary and mobile phases is discussed, and capacity factors are reported for 14 drugs on the three columns with three aqueous acetonitrile-based mobile phases. The fully automated technique (flow scheme given) permits the determination of both polar and apolar sulfonamides as well as dapsone in concentration. 5 to 20 µg kg-1, with coefficient of variation of 4 to 10% and recoveries of 85 to 90%.
HPIC Spectrophotometry Post-column derivatization

"Improved Detection Limits For Electrospray Ionization On A Magnetic Sector Mass Spectrometer By Using An Array Detector"
J. Am. Soc. Mass Spectrom. 1994 Volume 5, Issue 3 Pages 194-200
Robert B. Cody*, Jun Tamura, Jeffrey W. Finch and Brian D. Musselman

Abstract: The MS detection limits were investigated of hen egg-white lysozyme, equine myoglobin and ubiquitin on a JEOL JMS-HX110A forward-geometry double-focusing mass spectrometer equipped with an electrospray ionization source (cf. Anal. Chem., 1992, 64, 1561) and an array detector system similar to that described by Hill et al. (cf. Int. J. Mass Spectrom. Ion Proc., 1991, 111, 1). Samples were dissolved in aqueous 50% methanol containing 2% acetic acid and introduced into the source by infusion (2 µL/min) or by flow injection via a 20 µL loop injector. Detection limits with the array detector were at least 10 times lower than those that could be achieved by use of a point detector with the same MS. For hen egg-white lysozome the minimum detectable quantity at a signal-to-background ratio of 2:1 was 500 a mol/µL of solution However, the ultimate practical sample concentration appeared to be 10^-100 fmol/µL for the analysis of dilute solution of relatively pure proteins or simple mixtures.
Proteins Mass spectrometry

"A Flow Injection System For Studying Heat Inactivation Of Listeria Monocytogenes And Salmonella Enteritidis In Liquid Whole Egg"
J. Food Prot. 1996 Volume 59, Issue 2 Pages 121-126
Muriana, Peter M.; Hou, Huiying; Singh, Rakesh K.

Abstract: A flow injection system was devised to mimic continuous-flow-through pasteurization systems for laboratory thermal inactivation studies. Air bubbles were introduced into the sample stream to create separate moving segments (plugs) of liquid stream during pasteurization while residence time was adjusted by a combination of pump speed and column length. The method was used to obtain thermal inactivation data for Listeria monocytogenes Scott A and Salmonella enteritidis ATCC 13076 in liquid whole eggs at different temperatures and heating times. Thermal inactivation of L. monocytogenes using the capillary tube method (Z-cap = 7.3°C) gave results comparable to those obtained with the flow injection system (Z-flow = 7.2°C). The flow injection system also was used to examine thermal inactivation of S. enteritidis (SE) grown in either tryptic soy broth (TSB) or egg yolk medium (EYM) before inoculation into liquid whole egg (LWE). D-values were obtained by regression analysis and the data showed that SE grown in EYM gave D-values 15 to 120% higher than those obtained for SE grown in TSB. Thermal inactivation studies performed with S. enteritidis grown in commercial broth media may therefore inaccurately represent thermal resistance of S. enteritidis grown in liquid or shell raw egg as may occur in egg-associated outbreaks. The continuous-flow injection system described herein may be adapted to study continuous-flow pasteurization processes not easily examined by the traditional capillary tube method.
Enzyme, activity Process monitoring

"Comparison Of Four Wet Digestion Procedures For The Determination Of Selenium In Eggs By Hydride Generation-flow Injection Atomic Absorption Spectrometry"
Shipin Yu Fajiao Gongye 1987 Volume 4, Issue 4 Pages 7-9
Sun Junyan

Abstract: A comparison was made between four different wet digestion methods for the determination of Se in eggs by hydride generation AAS using a flow injection system. The coefficient of variation for the HNO3-HClO4 HNO3-(NH4)2S2O3 and HNO2-pr-essurized teflon bomb methods were, 6.7-9.9%, 7.4% and 7.6% respectively, and the percentage recoveres were 90-105%, 92% and 97% respectively. The HNO3-H2SO4 method was not able to decompose the organic material completely, and the sample was easily carbonized giving low results.
Selenium Sample preparation Spectrophotometry