University of North Florida
Browse the Citations
-OR-

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

View Stuart Chalk's profile on LinkedIn

Silk fibroin membrane

Classification: Membrane -> Silk fibroin membrane

Citations 3

"Amperometric Biosensor For Uric Acid Based On Uricase-immobilized Silk Fibroin Membrane"
Anal. Chim. Acta 1998 Volume 369, Issue 1-2 Pages 123-128
Yu-Qing Zhang*, Wei-De Shen, Ren-Ao Gu, Jiang Zhu and Ren-Yu Xue

Abstract: An amperometric urate sensor based on a uricase-immobilized silk fibroin membrane and an O electrode in flow injection analysis are described. The biosensor shows that recoveries of uric acid in human serum and urine are at 94.2-102.6% and 92.5-97.9%, respectively. The relative standard deviations (RSDs) for repeatedly monitoring standard urate solution, human serum and urine are 2.37, 3.72 and 2.95%, respectively., based on 100 measurements. The urate sensor based on the uricase-immobilized membrane is capable of detecting 60-70 human serum samples per h. Also, a piece of uricase-immobilized fibroin membrane used at the sensor could not only be stored for over 2 yr, but also repeatedly monitored >1000 times for biosamples such as human serum or urine.
Uric acid Serum Human Urine Sensor Amperometry Electrode

"Natural Silk Fibroin As A Support For Enzyme Immobilization"
Biotechnol. Adv. 1998 Volume 16, Issue 5-6 Pages 961-971
Yu-Qing Zhang

Abstract: A review with 40 references. Silk fibroin derived from Bombyx mori cocoon is being developed and utilized for purposes besides traditional textile material. Fibroin can be easily made up into various forms, several of which can serve as enzyme-immobilized supports. There are numerous reports on immobilized enzymes using these forms of silk fibroin as supports in which the enzyme-immobilized fibroin membranes were characterized in detail by means of spectrophotometry, IR spectra, NMR, ESR. Enzyme-immobilized fibroin membranes have been successfully used in several biosensors for the determinations of glucose, hydrogen peroxide and uric acid in which glucose and urate biosensors in a flow injection system were able rapidly to analyze various biosamples including human whole blood or serum.
Glucose Hydrogen peroxide Uric acid Serum Human Whole Human Sensor

"Characterization Of Uricase-immobilized Silk Fibroin Membrane And Its Application To Biosensor For Uric Acid"
Shengwu Huaxue Yu Shengwu Wuli Jinzhan 1998 Volume 25, Issue 3 Pages 82-85
ZHANG Yuqing, GU Renao, ZHU Jiang, CAO Peigen

Abstract: Uricase-immobilized silk fibroin membrane was characterized by means of electrochemical method. When uricase was immobilized in silk fibroin membrane, the immobilized uricase was improved largely at its stability to heat and pH, and it an be stored for long time. Based on this uricase membrane and oxygen electrode, an amperometric sensor has been developed for measurement of uric acid in flow injection analysis. The biosensor has a good reproducibility for repeatedly monitoring biosamples for 100 times respectively and is capable of detection over 60 serum samples per h.
Uric acid Blood Serum Sensor