University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Jet ring cell

Classification: Manifold component -> Jet ring cell

Citations 13

"A Bead Injection System For Copper Determination"
Anal. Lett. 2000 Volume 33, Issue 5 Pages 929-940
Claudio C. Oliveira; Elias A. G. Zagatto; Jaromir Ruzicka; Gary D. Christian

Abstract: A bead injection system for copper determination is proposed. Chelex-100 resin beads are introduced in the system as a suspension that is trapped in the Jet Ring Cell. The passage of the sample zone by the beads promotes the sorption of Cu(II). When the colorimetric reagent (APDC) perfuses the beads it reacts with copper ions, forming a colored complex that is monitored at 436 nn. After the measurement, the spent beads are sent to waste and a new portion of fresh beads is trapped in the system. The bead injection system is versatile and can be used to concentrate analyte in different sample volumes, permitting determinations of a wide range of copper concentrations. The detection limit is 0.5 µg L-1 with a 500 µL sample, and 1.2 µg L-1 a 100 µl sample.
Copper(II) Water Spectrophotometry

"Sequential Injection Analysis"
Chem. Listy 1999 Volume 93, Issue 6 Pages 354-359
H. Pasekova, M. Polasek, and P. Solich

Abstract: The review deals with the principles and practical applications of the SIA (sequential injection analysis) techniques. The article involves 101 references covering the period from 1990 to 1998.

"Flow Injection Renewable Surface Techniques"
Anal. Chim. Acta 1995 Volume 308, Issue 1-3 Pages 14-19
Jaromir Ruzicka

Abstract: The principles of flow injection - renewable surface techniques are presented. The technique uses polymer particles, such as those used in chromatography, as reagent carriers. A small amount of the polymer was injected into the carrier stream and deposited in a jet-ring cell prior to injection of the analyte. Two examples of this technique ware described, namely: (i) the competitive immunoassay of the herbicide imazethepyr with fluorimetric detection; and (ii) the determination of Cr(VI) with reflectance spectrophotometric detection. For (i) imazethepyr competes with fluorescein-labelled imazethepyr for antimidazine mAb attached to Protein A conjugated beads. The calibration range was 5-5000 ng/ml and the RSD was 5% at the 2500 ng/ml level. For (ii) the reagent 1,5-diphenylcarbazide was adsorbed on to Polysorb C-18 beads. The calibration graph for Cr(VI) was linear for 10^-200 ng/ml at 540 nm and with a 500 µL injection volume.
Chromium Imazethapyr Spectrophotometry Fluorescence

"Sequential Injection Analysis For Electrochemical Measurements And Process Analysis"
Analyst 1994 Volume 119, Issue 11 Pages 2309-2314
Gary D. Christian

Abstract: An overview is presented on sequential-injection analysis (SIA). The principles of SIA and some of its operational parameters are briefly described. Examples are provided to show that SIA is a versatile solution handling system for electrochemical and process systems. The examples given include coulometric titration with automated dilution, potentiometric studies using the matched potential method for assessing relative potentiometric selectivity coefficients, and the incorporation of the jet ring (fountain) cell in an SIA manifold for development of renewable reaction surfaces and sensors. (40 references).
Electrochemical analysis Coulometry Potentiometric stripping analysis

"Flow Injection Renewable Fibre Optic Sensor System. Principle And Validation On Spectrophotometry Of Chromium(VI)"
Analyst 1995 Volume 120, Issue 7 Pages 1959-1962
Oleg Egorov and Jaromir Ruzicka

Abstract: The central component of the cited sensor system consists of a jet-ring cell (diagram given) aligned with a multistrand bifurcated optical-fiber cable, which is incorporated in a sequential injection system. The renewable sensor system is based on the injection of a suspension of beads, on which the reagent is adsorbed, into a carrier stream. The analyte is then injected and is pre-concentrated on the beads, which are retained within the jet-ring cell. The reflectance is measured within the jet-ring cell. The reflectance is measured after which the spent beads are discharged from the system. The system was applied to the spectrophotometric determination of Cr(VI) using 1,5-diphenylcarbohydrazide as reagent and measuring the reflectance at 540 nm. The calibration graph was linear from 10^-200 ppb of Cr(VI); the detection limit was 9 ppb for a 500 µL injection volume. The RSD (n = 10) for 300 ppb of Cr(VI) was 1.1%.
Chromium(VI) Sensor Spectrophotometry

"Bioligand Interaction Assay By Flow Injection Absorptiometry Using A Renewable Biosensor System Enhanced By Spectral Resolution"
Analyst 1998 Volume 123, Issue 7 Pages 1617-1623
J. Ruzicka

Abstract: Conventional biosensors, such as those based on surface plasmon resonance, lack spectral resoln. and employ a permanent sensing layer that needs to be activated and also regenerated after use. In contrast, scanning of the UV/VIS spectrum of agarose beads, trapped in a specially designed flow cell, allows real time monitoring of labeled and unlabeled biomolecules, with spectral resoln., on a surface that can be automatically renewed, by microfluidic manipulation. Agarose beads are identical with column materials used in affinity chromatography and therefore are readily available, derivatized with a wide choice of bioligands. In this way, flow injection absorptiometry on renewable surfaces provides a basis for a new class of biosensors with 'open architecture' that allows the development of novel types of immunoassays employing both unlabeled and labeled molecules. The method also has interesting implications for affinity chromatography, because it uses identical materials and investigates the same type of bioligand interactions. An improved configuration of the jet ring cell is introduced and it is shown that both large (IgG) and small molecules (biotin) can be detected down to the 25 ng level reproducibly and rapidly.
Biotin Immunoglobulin G Sensor Spectrophotometry

"Jet Ring Cell: A Tool For Flow Injection Spectroscopy And Microscopy On A Renewable Solid Support"
Anal. Chem. 1993 Volume 65, Issue 24 Pages 3566-3570
Jaromir Ruzicka, Cy H. Pollema, and Kurt M. Scudder

Abstract: The cited flow-cell is described and illustrated. It exploits radial flow-through a narrow ring-shaped gap (20-50% of the particle diameter) to retain suspended particles within a well-defined detection region; trapped particles can be removed instantaneously by flow reversal. The cell was characterized by analysis of various beads (e.g., Polysorb MP1 and Sephadex) stained with dyes or fluorescent reagents and suspended in suitable buffers, using a sequential injection system with a flow-rate of 2.5 ml/min, a reverse flow-rate of 10 ml/min, a carrier solution of 0.01 M sodium borate buffer and a microscope system equipped with an epifluorescence attachment (details given). Cytodex 3 beads covered by adherent, stained BHK cells were also used with a carrier solution of HEPES buffer. The cell should prove useful for automated immunoassays and monitoring the pre-concentration of analytes on sorbents. A new flow cell design for spectroscopic measurements of suspensions, the jet ring cell, is introduced. This cell exploits radial flow-through a narrow ring-shaped gap to retain suspended particles within the detection region. This ring constitutes a detection volume of well- defined area from which the trapped particles can be instantaneously removed at will. The bed of particles thus forms a renewable surface, which can be probed by reflectance, fluorescence, or chemiluminescence using a microscope or optical fiber. This device should prove useful for microscopic study of cells, for automated immunoassays, and for pre-concentration of analytes on sorbents with in situ spectroscopic detection. In conjunction with a fiber optic detection system, the jet ring cell becomes a component of a renewable chemical sensor system.
Fluorescence Chemiluminescence Chromatography Immunoassay Microscopy

"Flow Injection Renewable Surface Immunoassay: A New Approach To Immunoanalysis With Fluorescence Detection"
Anal. Chem. 1994 Volume 66, Issue 11 Pages 1825-1831
Cy H. Pollema and Jaromir Ruzicka

Abstract: Agarose beads (~35 µm) coated with goat anti-mouse IgG1 (heavy chain specific) were diluted 1:20 in 0.01 M phosphate buffer containing 0.5 M NaCl (buffer A) to give a suspension containing ~2 x 105 beads/ml. Portions (42 µL) of the suspension were pumped into the jet ring cell of the sequential injection system (diagram given). The beads were retained on a optical flat surface monitored by fluorescence microscopy with excitation at 450-490 nm and use of a 520 nm long-pass emission filter. For competitive assays, a mixture of unlabelled (sample) and R-Phycoerythrin-conjugated mouse IgG1 mAb diluted in buffer A was then pumped into the cell. Unbound sample was washed away, the signal change due to the bound labelled sample was measured, and the beads were then removed from the cell with a reversed buffer flow; buffer A was used as the carrier throughout (0.25-1 ml/min). The calibration graph was linear from 1-5 µg/ml of mouse IgG1 mAb (using 5 µg/ml of labelled antigen and a contact time of 25 s). The method was also applied to a non-competitive immunoassay (details given).
Immunoglobulin G Plasma Mouse Fluorescence Immunoassay

"A Jet Ring Cell With A Renewable Solid Support For Amperometric Detection Of Glucose In A Sequential Injection Analysis System"
Anal. Lett. 1996 Volume 29, Issue 13 Pages 2257-2267
Tom Lindfors; Ilkka Lähdesmäki; Ari Ivaska

Abstract: A new amperometric method for the detection of glucose has been developed. A jet ring cell with a renewable solid support (JRR) is connected to a sequential injection analysis (SIA) system. The solid support, consisting of small spherical particles of agarose gel, with a mean bead size of 90 b.mum, is coupled with the enzyme glucose oxidase (GOD). The suspension is injected through a multiport valve in the SIA-system and is trapped in the JRR cell by a gap of 10 b.mum between the glassy carbon (GC) working electrode and a stainless steel counter electrode. The subsequent injection of glucose results in formation of hydrogen peroxide, which is detected by oxidation at 1.0 V (vs. Ag/AgCl/KCl (3 M)). Thereafter the suspension is removed by reversing the pump flow and subsequently replaced with fresh suspension for the next determination of glucose. The linear working range for the JRR-sensor, at this stage of the work, is 100 b.muM - 5 mM.
Glucose Amperometry

"Automated Solid Phase Extraction Of Theophylline By Sequential Injection On Renewable Column"
Anal. Commun. 1998 Volume 35, Issue 11 Pages 357-359
Brian Dockendorff, David A. Holman, Gary D. Christian and Jaromir Ruzicka

Abstract: A miniaturized and fully automated solid phase extraction system was developed based on sequential injection onto a renewable microcolumn of an ion exchanger. Extraction of theophylline from caffeine solutions was used as an example of sample preparation An important feature, compared to commercial sorbent extraction systems, was the elimination of prepackaged sorbent cartridges by using an automatically renewable microcolumn. The method has evolved from recent innovations in sequential injection analysis using a jet ring cell that entraps ion exchanger beads and discards them after completion of the monitoring cycle.
Theophylline Ion exchange

"Flow Detection System Using Chemical Sensor With Renewable Reaction Surface On A Solid Support"
Bunseki 1998 Volume 1998, Issue 11 Pages 881-883
Yamane, T.

Abstract: A review with 9 references is presented on jet-ring cell, application of flow injection renewable surface technology, and its future development.
Sensor

"Sequential Injection Systems Equipped With A Jet-ring Cell"
J. Flow Injection Anal. 1997 Volume 14, Issue 1 Pages 57-58
Nakako, S.

Abstract: NA

"Application Of Sequential-injection Analysis As Process Analyzers"
Lab. Rob. Autom. 1998 Volume 10, Issue 6 Pages 325-337
R. E. Taljaard, J. F. van Staden

Abstract: The development of sequential-injection analysis (SIA) from its mother technique flow injection analysis (FIA) is reviewed. A short historical background is given as well as discussions on the basic principles and operational parameters governing the design of an SLA system. Single-, double-, and multizone systems are described together with more complicated systems including calibration, dilution, extraction, dialysis, titrations, separation, pre-concentration, and systems incorporating mixing chambers.
d-Lactic acid Spectrophotometry