Contact Info
Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf
Potravinarske Vedy
- Publisher:
- FAD Code: PVVY
- CODEN: POVEEC
- ISSN: 0862-8653
- Abbreviation: Potravin. Vedy
- DOI Prefix: NA
- Other Name(s): Czech Journal of Food Sciences
- Language: Czech
- Comments: Fulltext from 2001 V19
Citations 1
"The Method Of Flow Injection Analysis And The Use Of Immobilized Glucose Oxidase: Glucose Determination In Food Products"
Potravin. Vedy
1991 Volume 9, Issue 2 Pages 81-88
Solich, P.;Karlicek, R.;Polasek, M.;Valentova, O.; Marek, M.
Abstract:
Glucose was determined in foods by flow injection analysis (FIA) combined with the use of immobilized glucose oxidase. H2O2, a product of glucose oxidase, was detected on the basis of its Mo-catalyzed reaction with I-. The I2 produced forms a blue complex with starch and is determined at 585 nm. The calibration graph is linear in the range 0.25-0.8 mM glucose, with a relative standard deviation of 1.53 for 8 measurements at a glucose concentration. of 0.51 mM. The FIA method was used to determine glucose in candies and cookies. Accuracy, sensitivity, and speed are advantages of the proposed method. (SFS)
Glucose
Food
Food
Spectrophotometry
Immobilized enzyme