University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Labor and Medizin

  • Publisher:
  • FAD Code: LBMD
  • CODEN: NA
  • ISSN: 0000-LBMD
  • Abbreviation: Labor Med.
  • DOI Prefix: NA
  • Language: German

Citations 2

"HPLC System With Post-column Derivatization For Fully Automated Determination Of 4-hydroxy-3-methoxymandelic Acid In Urine"
Labor Med. 1989 Volume 12, Issue 9 Pages 410-412
Wilmers, B.;Boos, K.S.;Sauerbrey, R.

Abstract: In the system described urine (adjusted to pH 3.0) was applied to a pre-column of C18-modified polyol copolymer. 4-Hydroxy-3-methoxymandelic acid (I) was eluted with buffer solution of pH 8.5 on to the analytical column (25 cm x 4.6 mm) of LiChrospher RP-Select B (5 µm). The eluate was passed to a reaction coil for post-column reaction at 60°C (cf. Pisano et al. Anal. Abstr., 1962, 9, 4363). The vanillin produced was determined at 345 nm.
Acids Urine HPLC Spectrophotometry Post-column derivatization Buffer Reactor Heated reaction

"Automated HPLC Analysis System For Direct Determination Of Catecholamines And Their Metabolites In Biological Matrices"
Labor Med. 1987 Volume 10, Issue 7-8 Pages 344-350
Boos, K.S.;Wilmers, B.;Sauerbrey, R.;Schlimme, E.

Abstract: This method for routine determination of adrenaline and noradrenaline in body fluids involves use of a laboratory-prepared SEC-HPAC (phenylboronic acid - vinyl polymer) column packing, a microprocessor-controlled column-switching technique and an integrated system for post-column derivatization and trihydroxyindole fluorescence monitoring. It permits direct injection of an appropriate biological fluid (500 µL) and has a detection limit of 2 pg.
Catecholamines Adrenaline Noradrenaline Biological fluid SEC Fluorescence Post-column derivatization