Abstract: The alkylating antitumor drugs carboquone, cyclophosphamide, thiotepa, busulfan, and melphalan in commercial preparations (powders, tablets, or injections) were dissolved in suitable solvents and determined by spectrometry with the flow injection system, using 4-(4-nitrobenzyl)pyridine as reactant and detection at 580 nm. This method is simple and fast, and the results are comparable with those from other methods (HPLC, gas chromatography, etc.). (SFS)

Abstract: Fluorometric determination of cyclophosphamide (I) [50-18-0] was performed by a manual method and flow-injection anal. with nicotinamide [98-92-0] and acetophenone [98-86-2]. In manual analysis, a 100 mL sample was treated with 500 mL nicotinamide solution (5%(w/v)) at 100°C for 60 min, and the reaction mixture was cooled, mixed with 500 mL acetophenone solution (2%(v/v)) and 500 mL KOH (0.5N in 50% EtOH), allowed to stand for 15 min and treated with 1.5 mL formic acid solution (60%) at 100°C for 3 min for the measurement of the fluorescence intensity (relative to standard quinine solution) at 440 nm with excitation at 385 nm. In the second analysis, a flow-injection system with FP110-type fluorometric detector was used. The calibration curve was linear in the range of 0.02-30 mg/100 mL for the manual analysis and of 0.98-16,000 ng/mL for the flow-injection analysis. The relative standard deviation was 2.37-5.16 and 1.23-3.30%, respectively. The methods were used in the determination of I in pharmaceutical preparations (injections, tablets). The flow-injection method was rapid (20 samples/h) and highly reproducible. (SFS)