University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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International Biotechnology Laboratory

  • Publisher:
  • FAD Code: ITBL
  • CODEN: IBLAEK
  • ISSN: 0888-7225
  • Abbreviation: Int. Biotechnol. Lab.
  • DOI Prefix: NA
  • Language: English

Citations 1

"Complete System For Fermentation Monitoring"
Int. Biotechnol. Lab. 1986 Volume 4, Issue 2 Pages 33-40
PARKER C. P. ; GARDELL M. G. ; DI BIASIO D.

Abstract: The Control Equipment Corporation MCA-103 MultiFlow Carbohydrate Analyzer is described. It is a fully automated monitoring device based on flow injection analysis with a gas-displacement system to deliver the solvents, and has two detectors, the first of which monitors the concentration. of a specific carbohydrate or alcohol with an integral detector by using immobilized oxidase enzymes and the second of which performs a broad range of chemical analyzes by using spectrophotometric detection. Its application is illustrated by the monitoring of glucose and phosphate levels in fermentation broths. Glucose was determined in the range 0.5 to 2.0% with glucose oxidase trapped between polycarbonate and cellulose acetate membranes stretched over a platinum anode. Phosphate (10 to 100 mg l-1) was determined by mixing the eluate from the glucose detector with molybdovanadate reagent and measuring the absorbance at 400 nm.
Carbohydrates Phosphate Glucose Fermentation broth Electrode Spectrophotometry Cellulose acetate Immobilized enzyme