Abstract: A solid-phase enzyme immunoassay of human IgG in the flow-injection mode is described. Samples of 20-50 mL of IgG solutions were injected into a polyethylene tubing leading 0.05 M phosphate buffer (pH 7.4, 0.14 M NaCl) at a flow rate of 0.5 mL/min to a 2 x 50 mm column packed with Sepharose 4B with BrCN-immobilized human IgG and immunologically bound rabbit antibody-horseradish peroxidase complexes. Peroxidase released into the eluate was mixed with H2O2 and ABTS substrates and the absorbance of the resulting colored product was measured in a flow cuvette at 420 nm. The contact between the samples and column packing (stopped flow) was varied 0.5-10 min; the contact between 0.5-2 min yielded almost linear calibration responses. The detection limits were 0.32 and 0.16 mg/mL for the contract time 0.5 and 10 min, respectively. The column allows ~100 samples to be analyzed without peroxidase complex reloading. Successive analyzes of the same sample show ~1% decrease in resulting values per run. The analyzes last 1-2 min each and have a coefficient of variation 3.2%. (SFS)

Abstract: A rapid method human IgG immunoenzyme determination based on the 'sandwich'-scheme of antigene concentration analysis is described. Both stages of this method are automated by using the techniques of flow injection analysis that are carried out in a kinetic regime. Antigene- antibody interaction kinetics were studied. The temporal ranges and concentrations of the employed reagents were determined. This allows to reduce the time of antigene-antibody interaction stages to 2-3 min. The proposed flow injection immunoenzyme method of human IgG assay with a chemiluminescence peroxidase detection system has a lower level of IgG determination 10^-8-10-9M. The total time of analysis is 5-10 min.