University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Journal of Molecular Recognition

  • Publisher: Wiley
  • FAD Code: JMLR
  • CODEN: JMORE4
  • ISSN: 0952-3499
  • Abbreviation: J. Mol. Recognit.
  • DOI Prefix: 10.1002/jmr
  • Language: English
  • Comments: Fulltext from 1988 V1

Citations 4

"Kinetic Study Of Site Directed And Randomly Immobilized His-tag Alkaline Phosphatase By Flow Injection Chemiluminescence"
J. Mol. Recognit. 2006 Volume 19, Issue 3 Pages 243-246
Juankun Zhang*, Anthony E. G. Cass

Abstract: We immobilized his-tag alkaline phosphatase (ALP) randomly and with the desirable orientation (site directed) to compare the effects of the enzyme activity on the beads. The chemiluminescence was employed to increase the sensitivity of enzyme labelled assays. Flow injection was also carried out for the detection of chemical and biological molecules in flow solutions. The Vmax of randomly immobilized his-tag ALP was 1.2 and the Vmax of site directed immobilized his-tag ALP was 1.5. In other words, the activity of site directed immobilized his-tag ALP was about 1.3-folds increased. The detection limit was detected to be 6 x 10^-6 M for the flow injection system.

"New Technologies For Amperometric Biosensors"
J. Mol. Recognit. 1996 Volume 9, Issue 5-6 Pages 664-671
Martyn G. Boutelle *, H. Allen, O. Hill, Manfred Berners, R. John, P. D. Dobson, P. Leigh

Abstract: Amperomeric-based detectors have successfully been used as personal monitors for blood glucose levels. However, there is a desire to increase the number of compounds measured in a small blood sample, the speed of detection and enhance the reliability of the measurement. Furthermore, with the increasing use of microdialysis as a clinical sampling method in metabolic medicine, paediatric medicine and neurointensive care, there is a need for rapid online detection of analytes such as lactate, glucose and glutamate in low microlitre volume samples. Two approaches to these problems are described. The first uses enzymes immobilized in a packed bed with electrochemical detection of a ferrocene mediator as a flow injection assay for use with microdialysis. Results from microdialysis of the brain of freely moving rats are described. In the second approach, thin-film techniques are used to fabricate arrays of microdisk and microline electrodes. The properties of these arrays in free solution and in a flow cell are presented together with an example using multiple arrays to identify an analyte by oxidation potential. Finally, different enzymes are entrapped onto the surface of two arrays by electrochemical polymerization of o-phenylenediamine. The resulting device detects glucose and lactate in real-time.
Glucose Glutamate Lactate Blood Amperometry Sensor Dialysis

"Biosensors In Flow Injection Systems For Biomedical Analysis, Process And Environmental Monitoring"
J. Mol. Recognit. 1996 Volume 9, Issue 5-6 Pages 658-663
Canh Tran-Minh

Abstract: This paper presents the construction of various biosensors using thin- film layers incorporated in flow injection devices, providing automated systems for biomedical analysis, process and environmental monitoring. A urease sensor has been developed in conjunction with a flow injection system for the automatic determination of urea. Use of the spraying immobilization technique gives rise to a response time of a few seconds, which allows sample throughputs up to 200 h-1. With a penicillin biosensor adapted in an appropriate cell detection, online measurements of penicillin V in the fermentation broth are achieved during the whole fermentation process; the results are compared with the HPLC method. Linearity, sensitivity and reproducibility of the biosensor are studied with regards to sample dilution in a stirred flow detection cell to provide optimal operating conditions. Measurements without any change in parameters are obtained during the whole fermentation process. Acetylcholinesterase sensors have been used in batch systems for the determination of pesticides, but they require large amounts of substrate. When those enzyme sensors are combined with flow injection systems, only small volumes (100 µL) of substrate are injected into the carrier stream and an automated system can be obtained for continuous control of water quality.
Urea Penicillin V Environmental Fermentation broth Sensor Process monitoring

"Principles And Applications Of Flow Injection Analysis In Biosensors"
J. Mol. Recognit. 1996 Volume 9, Issue 5-6 Pages 316-325
Elo Harald Hansen

Abstract: In practical applications biosensors are often forced to operate under less than optimal conditions. Because of their construction, and the physical processes and chemical reactions involved in their operation, compromise conditions are frequently required to synchronize all events taking place. Therefore, and in order to implement functions such as periodic calibration, conditioning and possible regeneration of the biosensor, and, very importantly, to yield the freedom to select the optimum detection means, it is advantageous to use these devices in a flow-through mode, particularly by employing the flow injection (FI) approach. The capacity of FI, as offering itself as a complementary facility to augment the performance of biosensors, and in many cases as an attractive alternative, is demonstrated by reference to selected examples, comprising assays based on enzymatic procedures with optical and thermal detection procedures, and via description of a recently introduced technique for immunoassays, termed flow injection renewable surface immunoassays, which promises to entail powerful potentials and to yield compatible or better economy of operation than existing approaches.
Immunoassay Sensor Theory Optimization Renewable surface