University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Shengwu Huaxue Yu Shengwu Wuli Jinzhan

  • Publisher: Wanfang Data
  • FAD Code: SWHY
  • CODEN: SHYCD4
  • ISSN: 1000-3282
  • Abbreviation: Shengwu Huaxue Yu Shengwu Wuli Jinzhan
  • DOI Prefix: 10.3724/SP.J.1206
  • Other Name(s): Progress in Biochemistry and Biophysics
  • Language: Chinese
  • Comments: Fulltext from 1999 V27

Citations 4

"Ultra-weak Chemiluminescence Analytical Technology Principle And Application"
Shengwu Huaxue Yu Shengwu Wuli Jinzhan 2000 Volume 27, Issue 4 Pages 441-442
ZHANG Zhong-Lun

Abstract: Ultra-weak chemiluminescence analytical technology has been developed rapidly in recent years. The technique in pharmaceutical analysis was introduced by using two examples. One is based on the chemiluminescence reaction of Ce (IV) -analginrhodamine 6G and the other is based on the auto-oxidation of analgin in micellar medium. An ultra-weak chemiluminescence analyzer has been coupled to flow-injection device for the determinations.

"Characterization Of Uricase-immobilized Silk Fibroin Membrane And Its Application To Biosensor For Uric Acid"
Shengwu Huaxue Yu Shengwu Wuli Jinzhan 1998 Volume 25, Issue 3 Pages 82-85
ZHANG Yuqing, GU Renao, ZHU Jiang, CAO Peigen

Abstract: Uricase-immobilized silk fibroin membrane was characterized by means of electrochemical method. When uricase was immobilized in silk fibroin membrane, the immobilized uricase was improved largely at its stability to heat and pH, and it an be stored for long time. Based on this uricase membrane and oxygen electrode, an amperometric sensor has been developed for measurement of uric acid in flow injection analysis. The biosensor has a good reproducibility for repeatedly monitoring biosamples for 100 times respectively and is capable of detection over 60 serum samples per h.
Uric acid Blood Serum Sensor Immobilized enzyme Silk fibroin membrane Process monitoring

"Application Of Immobilized Protein A In Flow Injection Fluoriimmunoassay"
Shengwu Huaxue Yu Shengwu Wuli Jinzhan 1995 Volume 22, Issue 1 Pages 81-84
Ren Xuezhen, J. N. Miller

Abstract: A convenient, semi-automated flow injection fluoroimmunoassay method has been developed incorporating the flexibility of sample hand1ing of flow injection system containing a immobilized protein A immunoreactor. Protein A can binds the Fc region of most mammlian antibodies at near neutral pH, and the bound antibodies can be eluted from protein A at acid pH. This peculiar property of protein was exploited to separate the antigen bound to antibody and the unbound ones. Experimental variables have been studied and the method has been used to determine the transferrin contents in human serum.
Transferrin Serum Human Immunoassay Fluorescence Immobilized enzyme

"Maltose Determination By A Multi-enzyme Electrode In A Flow Injection System"
Shengwu Huaxue Yu Shengwu Wuli Jinzhan 1995 Volume 22, Issue 1 Pages 72-76
Qu Hongbo, Zhang Xianen

Abstract: Amyloglucosidase was co-entrapped with glucose oxidase (GOO) in a poly vinyl alcohol membrane. The multi-enzyme membrane was combined with an oxygen electrode to form a bienzyme electrode, which could be incorporated in a flow injection system to determine maltose. Temperature effect and pH effect on the bienzyme electrode system were investigated. The linear respones range for the multi-enzyme electrode was 0.5-35 mmol/L of sugar. The entire response cycle was less than 2 min. The variation coefficient (CV) was 1.8%. The bienzyme electrode was used semi-continuously for 10 days, at the end of which period its respones to maltose remained 60% of its initial value. Methods to extend the life time of the bienzyme electrode and to eliminate the interference of coexisting glucose are discussed.
Maltose Electrode Interferences Optimization