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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
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Shengwu Gongcheng Xuebao

  • Publisher: Wanfang Data
  • FAD Code: SGXB
  • CODEN: SGXUED
  • ISSN: 1000-3061
  • Abbreviation: Shengwu Gongcheng Xuebao
  • DOI Prefix: 10.13345/j.cjb
  • Other Name(s): Chinese Journal of Biotechnology
  • Language: Chinese
  • Comments: Fulltext from 2000 V16

Citations 6

"Biosensor Based On Chemiluminescence For Serum Uric Acid Determination"
Shengwu Gongcheng Xuebao 1995 Volume 11, Issue 3 Pages 233-238
Liu Jianguo Guo Jian Li Gaoxiang

Abstract: A biosensor based on luminol chemiluminescent reaction and immobilized uricase column for serum uric acid determination has been developed. The response time by the sensor for various concentrations of serum uric acid was 47 sec with a 17 µL sample volume at 40 samples per hr. The linear range of standard curve was from 1 mg/dL up to 20 mg/dL of serum uric acid. The imprecision within a day was 3.22% to 4.36%. The day-to-day imprecision was 6.18% to 7.80%. The recovery rate by the method was 93% to 109%. Compared with the standard colorimetric method employed, the enzymatic kit revealed that the linear regression and correlation coefficient were Y = 0.4 + 0.938x and r = 0.9909, respectively. The immobilized uricase column retained 94% of its original activity even after over 2000 runs for five and half months of continual usage.
Uric acid Blood Serum Chemiluminescence Sensor Immobilized enzyme Column

"Determination Of L-glutamate Using Flow Injection Analysis With Immobilized L-glutamate Oxidase Reactor"
Shengwu Gongcheng Xuebao 1994 Volume 10, Issue 4 Pages 351-355
Li Qingshan Ye Bangce Zhang Siliang Yu Juntang

Abstract: L-Glutamate oxidase (GOD) and horseradish peroxidase (HRP) were covalently coupled on alkylamine pretreated controlled pore glass (CPG) by means of glutaraldehyde. The immobilized enzymes were packed into a teflon tube and used in flow injection analysis (FIA) system for L- glutamate determination. A good linearity range was obtained at 0.1-2.0 mM, and the coefficient of variation was 0.7% (n = 8). More than 80 samples were measured within an hour. The stability of the immobilized GOD reactor was good, retaining 50% of its initial activity after 4 months storage in buffer at 4°C. When the concentration of L- glutamate remained lower than 2.5 mM, the determination of L-glutamate in this system was not affected by pH and temperature within the range of 6.0-8.0 and 20-35°C, respectively. The system was applied to determine L-glutamate in broth samples during L-glutamate fermentation and good correlations were achieved between results obtained with the FIA system, L-glutamate oxidase kit and Warburg's method.
l-Glutamate Fermentation broth Immobilized enzyme Method comparison Controlled pore glass

"The Glutamate Biosensor And Its Application To Flow Injection Analysis System"
Shengwu Gongcheng Xuebao 1994 Volume 10, Issue 2 Pages 83-89
Ye Bangce Li Qingshan Li Yourong Yu Juntang

Abstract: A micro-enzyme electrode was fabricated by cross-linking L-glutamate oxidase with glutaraldehyde on aminopropyl-platinized platinum wire. A flow injection analysis system with glutamate sensor was used for L- glutamate determination. The peak current is linearly related to the L- glutamate concentration in the range of 0.02-2.0 mM, with good performance, accuracy (CV = 0.4%), fast response (< 60s), and stability (> 20 days). The system was applied to determine the concentration of L- glutamate in a fermentation broth. The recovery rate was in the range of 98.7-107.5%.
l-Glutamate Fermentation broth Sensor Electrode Apparatus

"Study Of The Enzyme Electrode Based On Glutamate Oxidase"
Shengwu Gongcheng Xuebao 1993 Volume 9, Issue 3 Pages 277-281
Yang Qingling, Bi Kewan

Abstract: A diffusion-controlled enzyme electrode was constructed for L-glutamate determination. The glutamate oxidase was immobilized between the cellulose acetate membrane and polycarbonate membrane using serum albumine and glutaraldehyde. The enzyme membrane was attached on the hydrogen peroxide probe surface moistened with electrolyte. The linear range of the enzyme electrode extends up to 1000 mg/L. A FIA system using the enzyme electrode was developed. The system exhibited good linearity (5-8000 mg/L with r = 0.9998), rapid response time (less than 20 s, and good selectivity. The electrode can be used continually for more than 2 weeks. The coefficient of variation (CV) of 41 times measurements at the glutamate concentration of 4000 mg/L is 2.8%. So its application to fermentation process control and food analysis is very promising.
Glutamate Fermentation broth Food Electrode Process control

"A Flow Dialysis Cell For Online Measurement Of Glucose In Fermentation Broth"
Shengwu Gongcheng Xuebao 1993 Volume 9, Issue 3 Pages 210-215
Zhang Xianen Hu Weiping Zhao Guoqiang Zhang Zhiping Zhang Xiaomei Gui Yiqun Zhang Xin Wei Hongping

Abstract: A plate flow dialysis cell, employing an acetate cellulose ultrafiltration membrane, was designed and tested for applicability to online sampling in fermentation. The glucose contained in effluents of both sample stream channel (Channel A) and carrier stream channel (Channel B) was determined with an enzyme electrode flow injection analysis system. Glucose penetration rate was defined as Rp, Rp = Gb/(Ga + Gb), here Ga and Gb are glucose concentrations of effluent of channel A and B respectively. A higher penetration rate was obtained when using phosphate buffer (0.01 M) as carrier solution instead of using distilled water. Operating pressure differences, temperature and residential time affected glucose penetration. Under the condition of 0.02 MPa pressure differences and 0.23 min of residential time (12.8 mL/L), Rp was about 12% in the range of 10^-70 mM with CV <4%. When sample stream was yeast broth, the glucose penetration rate Rp was stable for at least 48 hr. Good relationship was observed between online and off-line sampling for glucose determination in yeast fermentation, the correlation coefficient r was 0.985.
Glucose Fermentation broth Dialysis

"Study On Multiple-enzyme Electrode For Sucrose Determination"
Shengwu Gongcheng Xuebao 1991 Volume 7, Issue 4 Pages 339-344
Hu W, Zhang X, Zhang X, Hu S.

Abstract: Invertase (INV), mutarotase (MUT), glucose oxidase (GOD) and BSA were coimmobilized via glutaraldehyde-bridged covalent bonding, and directly absorbed on the teflon membrane. This membrane was covered with a nylon mesh and placed over an oxygen electrode. An enzyme electrode for flow injection analysis system (EFIA) was adopted. The optimum enzyme composition (IU) for immobilization on the teflon membrane of INV-MUT-GOD was found to be in the ratio 72:48:2.4, with a recovery activity INV-MUT of more than 42.9%. pH 5.8-6.5 was the most suitable range of acidity for the sensor activity. The optimum temperature was 35-45°C. The system exhibited good linearity in the range of 5 x 10^-4 approximately 10^-1 M sucrose (kinetic method) and 10^-5 approximately 2 x 10^-3 M sucrose (steady state method), in short response time (20 seconds for kinetic method, 2 minutes for steady state method), CV = 1.7% (kinetic method). The sensor had been used for determining sucrose concentration in fermentation broth, with an average recovery rate of 98%. The interference caused by the presence of glucose derived from decomposition of sucrose was eliminated by calibration with a GOD sensor. No significant loss of the enzyme electrode activity was observed after 120 hours of the continuous-flow of fresh 1 mM sucrose. The multiple-enzyme membrane showed a relatively long lifetime (compared with 14 hours as reported previously) and good storage stability (30 days, stored in distilled water at 4°C).
Sucrose Fermentation broth Electrode Heated reaction Immobilized enzyme Interferences Kinetic pH Teflon membrane Steady state