University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Preparative Biochemistry and Biotechnology

  • Publisher: Taylor and Francis
  • FAD Code: PBBT
  • CODEN: PBBIF4
  • ISSN: 1082-6068
  • Abbreviation: Prep. Biochem. Biotechnol.
  • DOI Prefix: 10.1080/1082606
  • Language: English
  • Comments: Fulltext from 2001 V31

Citations 3

"Simultaneous Detection Of Creatine And Creatinine Using A Sequential Injection Analysis/biosensor System"
Prep. Biochem. Biotechnol. 2006 Volume 36, Issue 4 Pages 287-296
Raluca-Ioana Stefan-van Staden; Rahel Girmai Bokretsion; Jacobus F. van Staden; Hassan Y. Aboul-Enein

Abstract: Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxide has been detected amperometrically. The linear concentration ranges are of pmol/L to nmol/L magnitude, with very low limits of detection. The proposed SIA system can be utilized reliably for the on-line simultaneous detection of creatine and creatinine in pharmaceutical products, as well as in serum samples, with a rate of 34 samples per hour and RSD values better than 0.16% (n = 10). Copyright © Taylor & Francis Group, LLC.

"Biosensors For The Determination Of Ortho-acetyl-L-carnitine. Their Utilization As Detectors In A Sequential Injection Analysis System"
Prep. Biochem. Biotechnol. 2003 Volume 33, Issue 3 Pages 163-172
Raluca-Ioana Stefan; Rahel Girmai Bokretsion; Jacobus F. van Staden; Hassan Y. Aboul-Enein

Abstract: In order to determine ortho-acetyl-L-carnitine, two biosensors were proposed. The biosensors were designed using physical immobilization of L-amino acid oxidase (L-AAOD) and horseradish peroxidase (HRP). Electrode characteristics were obtained and compared for the two carbon paste (graphite powder and paraffin oil) biosensors. The linear concentration ranges for the proposed biosensors were in the ranges of fmol/L to nmol/L, magnitude order with low limits of detection. Due to their reliability, the biosensors were used as detectors in a sequential injection analysis system, and gave reliable results for on-line assay of ortho-acetyl-L-carnitine in synthesis process control with a frequency of 75 samples per hour.

"Bienzymatic Amperometric Sensor For Protein Assay In Milk"
Prep. Biochem. Biotechnol. 2002 Volume 32, Issue 2 Pages 135-142
Stefan Raluca-Ioana, Makhapa A. Makhafola, Jacobus (Koos) F. van Staden

Abstract: A new bienzymatic amperometric sensor is proposed for the assay of the protein content of milk. The sensor is based on two enzymes: carboxypeptidase A and L-amino acid oxidase. The response characteristics obtained for this sensor (detection limit of 1.5 µmol/L, linear concentration range between 1.8 and 2.8 µmol/L) as well as high selectivity over possible interferences from milk. made it applicable as a detector in flow injection analysis (FIA). The response characteristics obtained in the non-equilibrium conditions (FIA system) are: detection limit of 1.5 µmol/L and linear concentration range between 2 and 3.5 µmol/L. Without FIA. the average recovery of proteins from milk and milk products is 99.06±0.07% and, by utilization of FIA, it increased to 99.73±0.03. The sensor proved a good reliability for the assay of proteins in milk and milk products.