University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Life Sciences

  • Publisher: Elsevier
  • FAD Code: LFSC
  • CODEN: LIFSAK
  • ISSN: 0024-3205
  • Abbreviation: Life Sci.
  • DOI Prefix: 10.1016/j.lfs,10.1016/0024-3205
  • Language: English
  • Comments: Fulltext from 1962 V1

Citations 3

"Utilization Of In Vivo Ultrafiltration In Biomedical Research And Clinical Applications"
Life Sci. 2003 Volume 73, Issue 16 Pages 2005-2018
Gea Leegsma-Vogt, Elsa Janle, Stephen R. Ash, Kor Venema and Jakob Korf

Abstract: Ultrafiltration (UF) is a filtrate selection method with a wide range of biomedical and clinical applications, including detoxification of blood in hemodialysis and peritoneal dialysis. New is, however, the use of UF as a convenient in vivo sampling method that, for example, has been used in diabetics. Ultrafiltration avoids complicated and time-consuming recovery calculations that are necessary when using in vivo microdialysis, as recoveries of low molecular weight molecules are near 100%. The subcutaneously or intravenously placed UF probes have been studied for off-line sample analysis and for continuous on-line monitoring, in a wide variety of species, including dogs, rats, pigs and humans.This review discusses the potential of in vivo UF as a continuous tissue sampling technique in clinical research areas, and in several major biomedical applications including glucose and lactate monitoring and drug kinetic studies.

"L-DOPA Does Not Facilitate Nitric Oxide Production In The Rat Striatum And Substantia Nigra: In Vivo Microdialysis Study"
Life Sci. 1998 Volume 63, Issue 4 Pages 59-64
Kenichi Kashihara*, Kenichi Sakai, Konosuke Marui and Toshikiyo Shohmori

Abstract: Using in vivo microdialysis and flow injection, we evaluated the production of nitric oxide (NO) in the striatum and substantia nigra of freely moving rats in response to challenge doses of L-dihydroxyphenylalanine (L-DOPA) by measuring the NO metabolite levels of nitrate and nitrite. The dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC) concentrations also were determined in the same perfusates. Intraperitoneal injection of L-DOPA produced a significant, dose-dependent increase in the extracellular levels of dopamine and DOPAC in these areas, but did not modify the extracellular levels of the NO metabolites. An acute dose of L-DOPA does not appear to facilitate NO production in the rat striatum and substantia nigra.
Nitrogen monoxide Brain In vivo monitoring

"Liquid Chromatography - Luminescence Methods"
Life Sci. 1987 Volume 41, Issue 7 Pages 901-904
Kazuko Mori

Abstract: Catecholamines in alumina extracts of urine or plasma or in tissue homogenates were separated by HPLC on TSK-gel ODS-1207 with KH2PO4 - acetonitrile - EDTA as mobile phase, pre- or post-column derivatization and fluorimetric detection. After post-column reaction based on trihydroxyindole formation with Fe(CN)63- as oxidant, the fluorescence of adrenaline and noradrenaline derivatives was measured at 520 nm (excitation at 410 nm). Dopamine could not, however, be detected in plasma by this method or by an alternative technique with electrochemical detection. The method could easily be automated, including a pre-column system. By pre-column derivatization with 1,2-diphenylethylenediamine (after cleanup on a cation-exchange column), dopamine and the other catecholamines could be determined in plasma; the detection limit was ~2 fmol. Fluorescence was measured at 480 nm (excitation at 350 nm).
Catecholamines Adrenaline Noradrenaline Biological tissue Blood Plasma Urine HPLC Fluorescence Sample preparation Post-column derivatization Pre-column derivatization