University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Folia Pharmaceutica Universitatis Carolinae

  • Publisher:
  • FAD Code: FPUC
  • CODEN: FUPCEA
  • ISSN: 1210-9495
  • Abbreviation: Folia Pharm. Univ. Carol.
  • DOI Prefix: NA
  • Language: Czech
  • Comments: Abstracts form 1992

Citations 4

"Determination Of Dihydroxyphenols By Flow Injection Analysis (fia) With Chemiluminescence Detection"
Folia Pharm. Univ. Carol. 2005 Volume 33, Issue 1 Pages 67-75
Jana Dolejšová, Miroslav Polášek, Petr Solich, Rolf Karlí?ek

Abstract: Flow injection method with chemiluminescence detection has been devised for determining ≈2-8 µmol L-1 of dihydroxyphenols (catechol, resorcinol, hydroquinone). The samples are made 60 mmol L-1 in aqueous sodium hexametaphosphate (enhancer of the chemiluminescence) and injected (for 5 s) into a reagent stream (flow rate 0.91 mL min-1) of 0.25 mol L-1 H2SO4 containing 0.5 mmol L-1 KMnO4 (for catechol) or 0.3 mmol L-1 KMnO4 (for the other phenols) as oxidant. The injected sample volume was 200 µL. The relative standard deviation (RSD) values of the peak heights ranged betwen 1.74% (resorcinol) and 0.82% (hydroquinone), n = 10, when injected ≈5 µmol L-1 calibration solutions of the phenols. The method has been applied to the analysis of phenols as bulk substances. The sample throughput was 171 sample hr-1. The results were in good agreement with those of VIS spectrophotometric (Emerson reaction) determination, as evaluated by the Student t-test.

"Determination Of Trihydroxyphenols By Flow Injection Analysis (fia) With Chemiluminescence Detection"
Folia Pharm. Univ. Carol. 2005 Volume 33, Issue 1 Pages 57-65
Jana Dolejšová, Miroslav Polášek, Petr Solich, Rolf Karlí?ek

Abstract: Flow injection chemiluminescence method for determining ≈1-13 µmol L-1 of trihydroxyphenols (pyrogallol, phloroglucinol and 1,2,4-trihydroxybenzene) using the analyte oxidation in aqueous 0.3 mmol L-1 KMnO4 containing 0.25 mol L-1 H2SO4 as reagent stream (flow rate 0.91 mL min-1) has been devised. Sodim hexametaphosphate (60 mmol L-1) added to the injected phenol samples increased the intensity of chemiluminescence 1,2,4-trihydroxybenzene 4.5 fold relative to the sample without this enhancer of chemiluminescence. At the injected sample volume of 200 µL, the relative standard deviation (RSD) values of the peak heights was 1.02% (pyrogallol), 1.43% (phloroglucinol) and 0.68% (1,2,4-trihydroxybenzene), n = 10, when injected 5 µmol L-1 calibration solutions of phenols. The sample throughput was 171 sample hr-1. The results were in good agreement with those of VIS spectrophotometric (Emerson reaction) determination, as evaluated by the Student t-test. The proposed method has been applied to the analysis of phenols as bulk substances.

"Flow-Injection Spectrophotometric Determination Of Phenols Using Analyte Oxidation In Manganese Dioxide Or Lead Dioxide-Packed Reactors"
Folia Pharm. Univ. Carol. 2002 Volume 27-28, Issue 1 Pages 59-68
Jana Dolejšová, Miroslav Polášek, Rolf Karlí?ek

Abstract: Flow-injection spectrophotometric method for determining 0.2 to 400 mmol L-1 of dihydroxyphenols (catechol, resorcinol, hydroquinone) and of trihydroxyphenols (pyrogallol, phloroglucinol, 1,2,4-hydroxyhydroquinone) using the analyte oxidation in manganese dioxide or lead dioxide-packed reactors (50 mm x 1.2 mm) with aqueous 10 mmol L-1 H2SO4 as carrier flow (0.6 mL min-1) has been devised. Active and stable reactors packings were prepared by coating sintered glass particles (diameter 0.4 to 0.5 mm) successively with an epoxide glue and powdered MnO2 or PbO2. At the injected sample volume of 80 µL the relative standard deviation was 1.37 % (n = 10) when determining 1.0 mmol L-1 of catechol (8.8 µg in 80 µL of the solution) in prepared solution of the pure substance and the sample throughput was 90 h-1 (in manganese dioxide-packed reactors) or 60 h-1 (in lead dioxide-packed reactors). The method has been applied to the analysis of phenols as bulk substances. The results were in good agreement with those of pharmacopoeial UV spectrophotometric determination.

"Application Of Flow Injection Analysis In The Analysis Of Pharmaceuticals And Biologically Active Compounds"
Folia Pharm. Univ. Carol. 1998 Volume 21-22, Issue 1 Pages 53-64
Petr Solich

Abstract: A review with 19 references. The author's research in the development and application of flow injection analysis (FIA) of pharmaceuticals and biological active compounds is summarized. The review includes applications of FIA for the determination of active ingredients in mass-produced pharmaceutical preparations, determination of the total drug content, determination of the active ingredient content uniformity in individual tablets, etc. Future FIA methods will take advantage of flow fluorimetric detection, immobilized enzymes, pre-concentration, absorption on a solid phase, and biosensors.
Drugs Organic compounds Pharmaceutical Fluorescence Sensor Review Immobilized enzyme Preconcentration Solid phase extraction