University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Medical Laboratory Sciences

  • Publisher:
  • FAD Code: MDLS
  • CODEN: BJMSEO
  • ISSN: 0308-3616
  • Abbreviation: Med. Lab. Sci.
  • DOI Prefix: NA
  • Other Name(s): Medical Laboratory Technology, British Journal of Biomedical Science (0967-4845)
  • Language: English
  • Comments: Fulltext from 2002 V59

Citations 5

"Direct Determination Of Urinary Oxalate By A Continuous-flow Method"
Med. Lab. Sci. 1990 Volume 47, Issue 2 Pages 73-79
Goldsack K, Ginman RF, Wright JM

Abstract: The sample, in water as carrier, is mixed with succinate buffer of pH 5.6 and passed through a reactor containing ascorbate oxidase (to oxidize ascorbate, which otherwise interferes) immobilized (method described) on the inner surface of O-alkylated nylon tubing. The stream is then mixed with succinate buffer of pH 2.0 before passage through a reactor containing immobilized oxalate oxidase, and 3,5-dichloro-2-hydroxybenzenesulfonic acid - peroxidase reagent is added to react with the H2O2 thus formed. The absorbance of the product is measured at 510 nm. Response is rectilinear for up to 0.5 mM oxalate. The method shows good selectivity. At 0.3 mM oxalate, the within- and between-batch coefficient of variation were 1.4 and 1.7%, respectively (n = 10). Recovery of added oxalate was >91%. Results were well correlated with those of an enzymatic 3-methylbenzothiazolin-2-one hydrazone method.
Oxalate Urine Spectrophotometry Buffer pH Immobilized enzyme Selectivity Method comparison Interferences

"Bioluminescence In The Continuous-flow Analysis Of Creatinine Using Immobilized Creatinine Amidohydrolase [creatininase]"
Med. Lab. Sci. 1985 Volume 42, Issue 4 Pages 310-317
Colliss JS, Wright JM, Ginman RF

Abstract: Serum was cleaned-up by addition of Dowex-50 cation-exchange resin and 6.5 mM HCl and the mixture was centrifuged after 30 min. Creatinine(I) was eluted from the resin with 0.125 M phosphate buffer (pH 12). The eluate was mixed with a solution containing ATP, Mg acetate and creatine kinase, and passed through a column of immobilized creatininase. The resulting solution was mixed with luciferin - luciferase and the bioluminescence was measured. Urine samples were diluted with water before analysis. Recoveries were 98 to 110 and 61 to 65% for I in urine and serum, respectively, and the coefficient of variation were 6.72%. Calibration graphs were rectilinear for 600 µM and 1 mM for urine and serum, respectively.
Creatinine Blood Serum Urine Bioluminescence Ion exchange Resin Immobilized enzyme

"Automated Continuous-flow Analysis Of Total Protein In Urine"
Med. Lab. Sci. 1984 Volume 41, Issue 1 Pages 66-67
O'Malley AH, Penney MD

Abstract: The manual micro-turbidimetric method of Iwata and Nishikaze (Anal. Abstr., 1980, 38, 3D197) has been automated; a flow diagram is illustrated and reagent solution compositions are given. Within-run coefficient of variation are typically 2 to 6% in the range 0.15 to 1.79 g L-1 of protein, the corresponding between-run values being 6 to 8%; carry-over is 1.6%.
Protein, total Urine Turbidimetry

"Automated Capillary-blood-spot Glucose Estimation"
Med. Lab. Sci. 1979 Volume 36, Issue 4 Pages 379-380
West P, Marsland I, Bradshaw P.

Abstract: Capillary blood spots, collected by the diabetic patient onto filter paper, were dried and sent by mail to the lab. A 6-mm disk, containing 11 µL of blood, was punched from the spot and eluted into a sulfosalicylic acid Elution-fixer reagent. After 60 min, the glucose was analyzed by the AutoAnalyzer using the glucose oxidase method of P. Trinder (1969). Mean recovery of glucose added to blood was 97%; within-batch relative standard deviation was 4.9% for 12.9 mmol/L and 5.2% at 5.8 mmol/L. (SFS)
Glucose Blood Clinical analysis Technicon

"Simultaneous Automated Continuous-flow Analysis Of Total Oestrogen And Creatinine In Pregnancy Urine"
Med. Lab. Sci. 1979 Volume 36, Issue 3 Pages 293-296
Phillips, Susan D.

Abstract: Modifications of the methods of M. Lever et al. (1973) for estrogen and A. L. Chasson et al. (1961) for creatinine (I) are described. Estrogen standards were prepared by diluting the stock solution with urine from males; I standards were diluted with water. The diluted samples for I determination were taken from the estrogen waste-line and further diluted with water containing Brij-35 (30%) before reacting with alkaline picrate. This dilution decreases the need for dialysis of the urine and also decreases interferences by other substance. Any urines showing +++ or greater for glucose and(or) ketones, as determined by the Keto-diastix, were treated with octan-2-ol and NaBH4 (in glucose presence) and by boiling (in ketones presence). The through time for this automated determination is 11 min for I and 18 min for estrogens. (SFS)
Estrone Creatinine Urine Clinical analysis Interferences