University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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American Chemical Society Symposium Series

  • Publisher: Oxford University Press
  • FAD Code: CSSS
  • CODEN: ACSMC8
  • ISSN: 0097-6156
  • Abbreviation: Am. Chem. Soc. Symp. Ser.
  • DOI Prefix: 10.1021
  • Other Name(s): ACS Symposium Series
  • Language: English
  • Comments: Book series. Available from 1974 V1

Citations 4

"Study Of Bacterial Metal Resistance Protein-based Sensitive Biosensors For Heavy Metal Monitoring"
Am. Chem. Soc. Symp. Ser. 2000 Volume 762, Issue 1 Pages 102-112
Ibolya Bontidean, Jon R. Lloyd, Jon L. Hobman, Nigel L. Brown, Bo Mattiasson, and Elisabeth Csöregi

Abstract: A capacitive signal transducer was used with metal-resistance (SmtA) and metal regulatory (MerR) proteins to construct sensitive biosensors for monitoring heavy metal ions. The proteins were overexpressed in E. coli, purified and immobilized on a gold electrode modified with self-assembled thiol layers. The protein-modified electrode was used as the working electrode in an electrochemical cell placed in a flow injection system. Both the metallothionein from the cyanobacterium Synechococcus PCC 7942 and the MerR regulatory protein from transposon Tn501 enabled monitoring of Cu2+, Hg2+, Zn2+, and Cd2+ starting from femtomolar concentrations. The metal ions were bound differentially and the shape of the binding curves may sense conformational changes related to the biological roles of the proteins.

"FILIA Determination Of Imazethapyr Herbicide In Water"
Am. Chem. Soc. Symp. Ser. 2000 Volume 751, Issue 1 Pages 135-144
M. Lee, R. A. Durst, T. D. Spittler, and, D. R. Forney

Abstract: In response to the need for a rapid, economical method for determination of the herbicide imazethapyr at low concentrations in water, the capillary FILIA (flow injection liposome immunoanalysis) system has been applied. A capillary tube (57 cm ? 0.45 mm i.d.) with immobilized imazethapyr antibody was used as the immunoreactor column in the flow injection system. The assay is based on sequential competitive binding between imazethapyr and imazethapyr-tagged liposomes for a limited number of antibody binding sites. Subsequent rupture of the liposomes by injection of a detergent (n-octyl ?-D-glucopyranoside) releases carboxyfluorescein which elutes and is measured fluorometrically. Water samples from wells, lysimeters and run-off were collected from test plots and monitored watersheds following imazethapyr application at the Chesapeake Farms environmental research center (Chestertown, MD). Imazethapyr residues in water samples were concentrated 10 times by partitioning into methylene chloride, which was then evaporated. The residue was dissolved in TBS (Tris-buffered saline) solution and injected onto the immunocolumn. The analysis provides a limit of detection of 0.01 ?g/L and a working range of 0.02-10 ?g/L imazethapyr.

"Watershed Monitoring In Sustainable Agriculture Studies"
Am. Chem. Soc. Symp. Ser. 2000 Volume 751, Issue 1 Pages 126-134
T. D. Spittler, S. K. Brightman, M. C. Humiston, and D. R. Forney

Abstract: To obtain maximum efficiency and sensitivity in pesticide residue studies of various cropping systems on multiple watersheds at Chesapeake Farms, a Dupont environmental research center, a series of triazines and acetanalides were first analyzed by gaschromatography with electron capture detection (GC-ECD) at sensitivities selected to give the maximum number of direct readings with minimal dilutions (0.5-1.5 ppb). Samples having non-detects for specific compounds in the multi-residue series were individually reanalyzed by enzyme immunoassay at a sensitivity of 0.1 ppb. Two synthetic pyrethroids were also analyzed using similar GC-ECD parameters, and a separate flow injection liposomal immunoassay (FILIA) was developed for imazethapyr.

"Determination Of Organic Compounds In Aqueous Waste Streams By Online Total Organic Carbon And Flow Injection Analysis"
Am. Chem. Soc. Symp. Ser. 1992 Volume 508, Issue 1 Pages 62-78
W. W. Henslee, S. Vien, and P. D. Swaim

Abstract: Pollution Prevention in Industrial Processes. The determination of soluble organics in wastewater streams is important for efficient operation of biological treatment facilities, compliance with regulatory permits, detection of process upsets, and material balances around manufacturing units. Grab and composite samples, followed by TOC (total organic carbon) measurements in the lab. are often too slow to provide time for an appropriate response. Accuracy, precision, sensitivity and stability data are given for a commercial available TOC analyzer. In some cases, the time for a TOC measurement is too long for adequate response to a spill or process upset. Two cases are discussed in which flow injection analysis was used instead of TOC. Both involve the detection of amines in waste streams.
Amines Total organic carbon Industrial Total organic carbon Method comparison