Abstract: A high selectivity analytical method for p-hydroxymethamphetamine (pOHMA) in urine is presented. After extraction, the analyte was analyzed by high performance liquid chromatography with post-column oxidation using potassium hexacyanoferrate(III) and sodium hydroxide to detect the fluorophor of pOHMA. The recovery of pOHMA is 86.5%, and the calibration curve for pOHMA is linear in the range of 5-75 µg/mL (r2=0.997). The coefficients of variation determined for spiked samples are 2.2% for 10 µg/mL and 2.3% for 5 µg/mL pOHMA (each n=5), and the detection limit for pOHMA is 1 µg/mL (20 ng; S/N=3). Analysis for pOHMA in forensic samples is performed successfully, without interference from endogenous fluorophors, yielding concentrations in the appropriate range for methamphetamine abusers.