University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Tyrosine sulfate ester

@ ChemSpider@ NIST@ PubChem

Citations 2

"Analysis Of Sulfate And Phosphate Esters Of Amino-acids By Ion-exchange Chromatography On Polymeric DEAE"
J. Chromatogr. A 1985 Volume 327, Issue 1 Pages 9-15
David W. McCourt, Joseph F. Leykam and Benjamin D. Schwartz

Abstract: Chromatography was effected on a Waters Protein Pak DEAE-5PW column at 45°C with a linear gradient of 0 to 60% of 0.25 M H3BO3 - 0.05 M NaCl (pH 11) in propan-2-ol - 0.01 M H3BO3 (pH 11) (1:19). Post-column derivatization with phthalaldehyde facilitated fluorimetric detection of the amino-acids at 425 nm (excitation at 338 nm). This system satisfactorily separated the sulfate esters of serine and tyrosine and the phosphate esters of threonine, serine and tyrosine, and allowed the determination of tryptophan obtained by base hydrolysis of peptides and proteins, such as cholecystokinin-(26,33)-octapeptide.
HPIC Fluorescence Post-column derivatization

"Quantitation Of Tyrosine O-sulfate In Human Urine By Ion-pair Reverse-phase High Performance Liquid Chromatography"
Clin. Chim. Acta 1990 Volume 193, Issue 3 Pages 193-197
Masahito Suiko, P. H. Prasantha Fernando, Yoshio Arino, Masafumi Terada, Seiichiro Nakatsu and Ming-Cheh Liu,*

Abstract: Filtered urine was treated with Dowex 50W-X8 resin (c.f. Tallan et al., J. Biol. Chem., 1955, 217, 703). The supernatant solution was diluted 10-fold with water and 10 µL portions were analyzed by ion-pair HPLC on a column (25 cm x 4.6 mm) of Vydac C18 operated at 40°C, with 10% acetonitrile - 0.03 M KH2PO4 - 0.01 M tetrabutylammonium phosphate as mobile phase (1 mL min-1) and measurement of the absorbance at 261 nm or the fluorescence at 455 nm (excitation at 340 nm) following post-column derivatization with phthaldialdehyde (pH 10) in the presence of N-acetylcysteine. The calibration graph was rectilinear for 0.13 to 19 µg mL-1 of tyrosine-O-sulfate and down to 0.5 pmol could be determined. Recoveries were quantitative.
Urine HPLC Fluorescence Clinical analysis Column Post-column derivatization