Abstract: Many simple carbohydrates and other polyhydroxy compounds can be oxidized at a silver oxide surface. The oxidation is via an electrocatalytic mechanism involving a Ag(I) oxide. This forms the basis of a flow stream detector operated in an amperometric mode which may be used for either flow injection or high performance liquid chromatography (HPLC) applications. The title electrode has been applied to the detection of simple carbohydrates, triglycerides and nucleic acid components.

Abstract: The method is based on hydrolyis of neutral fat by triacylglycerol lipase (I) - carboxylesterase (II) and glycerol dehydrogenase (III)-catalyzed oxidation of glycerol, after which the NADH produced is monitored amperometrically. The cited sensor is made from I and II co-immobilized on aminopropyl-substituted controlled-pore glass and from III immobilized on Amino-Cellulofine. For application, an emulsion of milk in 6% bovine serum albumin solution is diluted with buffer solution prepared from 0.125 M carbonate buffer of pH 9.5 containing 30 mM (NH4)2SO4 and 1% of Triton X-100, and a portion is injected into the analyzer. (diagram given) for the determination of neutral fat at 25°C with the above-mentioned buffer as carrier stream at 1 mL min-1. When determining triolein, the relationship between response and concentration. is rectilinear for 0.0128 to 0.0853%; the coefficient of variation was 0.87%. Results were comparable with those obtained by the Wako triglyceride G-test method.