University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Spermidine

  • IUPAC Name: N'-(3-aminopropyl)butane-1,4-diamine
  • Molecular Formula: C7H19N3
  • CAS Registry Number: 124-20-9
  • InChI: InChI=1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
  • InChI Key: ATHGHQPFGPMSJY-UHFFFAOYSA-N

@ ChemSpider@ NIST@ PubChem

Citations 5

"Kinetic-photometric Determination Of Spermine, Spermidine And Their Mixtures By The Stopped-flow Technique"
Fresenius J. Anal. Chem. 1988 Volume 331, Issue 6 Pages 642-645
M. C. Gutiérrez, A. Gómez-Hens and D. Pérez-Bendito

Abstract: Kinetic photometric stopped-flow methods are described for the individual and simultaneous determination of two biogenic amines: spermine and spermidine. They are based on the colored reaction of these amines with sodium 1,2-naphthoquinone-4-sulfonate. The parameters used for the quantitative determinations are the initial rate and the increase in absorbance of the kinetic curves. Each amine can be rapidly determined in the range of 0.5-150 g ml-1 with a precision (%RSD) of less than 1.8. The simultaneous determination of mixtures of spermine and spermidine is carried out by the proportional equations method. Spermine/spermidine mixtures in ratios between 120 and 201 are successfully resolved.
Spectrophotometry Kinetic Stopped-flow

"Multi-enzyme-containing Tissue-based And Ferrocene-mediated Bioelectrode For The Determination Of Polyamines"
Electroanalysis 1992 Volume 4, Issue 5 Pages 521-525
Meng Shan Lin, Minoru Hare, Garry A. Rechnitz

Abstract: Ground oat seedling tissue (which contains polyamine oxidase and peroxidase) was mixed with 2 to 9% (w/w) of graphite - mineral oil (3:2) paste containing 2% of ferrocene, and the mixture was firmly packed into the cavity of a flow cell electrochemical detector or, for steady-state experiments, into the end of a tubular electrode (3 mm i.d.). The electrode tip was smoothed on paper. Polyamine oxidase catalyses the oxidation of polyamines, O as co-substrate is reduced to H2O2, and the peroxidase then oxidizes ferrocene to ferricinium at the expense of H2O2; the signal is monitored reductively at 0.0 V vs. Ag - AgCl. Steady-state amperometric and cyclic voltammetric experiments were carried out in conjunction with a Pt-wire counter electrode; for a working electrode containing 7% of oat seedling tissue, response to spermidine or spermine in 0.1 M phosphate buffer of pH 7 was rectilinear up to 15 or 7.5 µM, respectively, and the corresponding detection limits were 0.19 and 1.15 µM. In the flow injection analyzes, carried out with the same buffer, the Ag - AgCl reference electrode was placed in a compartment downstream of the flow cell, with a stainless steel auxiliary electrode downstream of this, and PTFE tubing was used. Response to spermidine or spermine was rectilinear up to 20 or 15 µM, respectively. The electrodes did not respond to injections of 0.1 µM-ascorbic acid, uric acid, histamine, 4-aminobutyric acid or agmatine.
Sensor Electrode Interferences

"High Performance Liquid Chromatographic Determination Of Polyamines In Selected Vegetables With Post-column Fluorimetric Derivatization"
J. Chromatogr. A 1993 Volume 628, Issue 2 Pages 199-204
Hideaki Ohta, Yuko Takeda, Koh-Ichi Yoza and Yoichi Nogata

Abstract: A method is described for the HPLC analysis of the aliphatic polyamines agmatine, putrescine, cadaverine, spermidine and spermine in vegetables. Sample (1 g) was homogenised with 7 mL of 5% perchloric acid, cooled for 1 h at 0°C, and centrifuged. The supernatant was analyzed on a column (3.5 cm x 6 mm) of Polyaminepak (strong cation-exchange resin, 5 µm) with a mobile phase (0.65 mL min-1) of 1.0 M sodium citrate (pH 5.4) - acetonitrile (9:1). Post-column derivatization with phthalaldehyde (details given) and subsequent fluorescent detection was used.
Vegetable HPLC Fluorescence Post-column derivatization

"High Performance Liquid Chromatographic Determination Of Plasma And Brain Histamine Without Previous Purification Of Biological Samples: Cation-exchange Chromatography Coupled With Post-column Derivatization Fluorimetry"
J. Chromatogr. B 1985 Volume 344, Issue 1 Pages 115-123
Atsushi Yamatodani, Hiroshi Fukuda and Hiroshi Wada, Toshinao Iwaeda, Takehiko Watanabe

Abstract: Plasma and homogenized brain were extracted with HClO4 and the extracts were analyzed directly by HPLC on a column (10 cm x 6 mm) of TSK gel SP-2SW (5 µm) with 0.25 M KH2PO4 as mobile phase (0.6 mL min-1). After the elution of histamine(I), spermidine and other strongly basic compounds were eluted with 0.5 M KH2PO4. I was determined fluorimetrically at 450 nm (360-nm excitation) after online post-column derivatization with phthalaldehyde. The detection limit was 0.05 pmol of I, and the calibration graph was rectilinear for 10 pmol. Recovery was 98.5% and the within- and between-day coefficient of variation were <3%.
Blood Plasma Brain HPLC Fluorescence Post-column derivatization

"Determination Of Polyamines By Flow Injection Analysis With A Chemiluminescence Detector Based On Their Complexation With Copper(II)"
Anal. Sci. 2006 Volume 22, Issue 5 Pages 763-767
Zheng-ping Li, Qiu-hua Wu, Chun Wang And Yu-qin Su

Abstract: Through the flow injection analysis experiments, we discovered that an unsaturated complex of Cu(II) and polyamines (spermine, spermidine, putrescine) had a strongly catalytic effect on luminol-H2O2 chemiluminescence (CL) reaction, and that the CL intensity is proportional to the concentrations of polyamines. Based on the automatic formation of an unsaturated complex of polyamines and Cu(II) when the solution containing polyamines passed through a column packed with solid Cu(OH)2, a new flow injection chemiluminescence analysis method was proposed for the determination of polyamines. The effects of pH, buffer concentration, the concentration of chemiluminescence reagent, and the influence of mixing coil length were examined. Under optimal conditions, the linear range was from 1.0 x 10^-7 mol L-1 to 1.0 x 10^-5 mol L-1, and the detection limits were 0.17, 0.38, 0.44 pmol for spermine, spermidine, and putrescine, respectively. Compared with other methods, the advantages of this method include convenience, time-saving and low cost.
Chemiluminescence Optimization Catalysis