University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Ribose

  • IUPAC Name: (3R,4R,5R)-oxane-2,3,4,5-tetrol
  • Molecular Formula: C5H10O5
  • CAS Registry Number: 50-69-1
  • InChI: InChI=1S/C5H10O5/c6-1-3(8)5(10)4(9)2-7/h1,3-5,7-10H,2H2/t3-,4+,5-/m0/s1
  • InChI Key: SRBFZHDQGSBBOR-SOOFDHNKSA-N

@ ChemSpider@ NIST@ PubChem

Citations 4

"Catalytic Oxidation And Flow Detection Of Carbohydrates At Ruthenium Dioxide-modified Electrodes"
Anal. Chem. 1990 Volume 62, Issue 14 Pages 1413-1416
Joseph Wang and Ziad Taha

Abstract: A RuO2-modified carbon paste electrode (prep. described) was used in the constant-potential cyclic voltammetric flow detection of carbohydrates. Samples in 1 M NaOH were analyzed at the electrode (+0.4 V; 20 mV s-1) with Ag - AgCl and Pt-wire reference and auxillary electrodes, respectively. The effects of variables, viz, pH, flow rate, operating potential and surface 'loading', were studied. The method was applied in the detection of deoxyribose, fructose, galactose, gluconic acid, glucose, glycerol, lactose, maltose, ribose and sucrose (electrode responses are tabulated). Working ranges were from 0.1 to 10 mM glucose and 0.1 to 1 mM ribose, fructose and galactose. The detection limits were pmol-levels and the coefficient of variation (n = 72) was 1.2%. The electrode was stable for >48 h with a signal loss of 10% over this period.
Electrode Electrode Voltammetry Detection limit Optimization Catalysis

"Postchromatographic Electrochemical Detection Of Carbohydrates At A Silver Oxide Electrode"
Electroanalysis 1993 Volume 5, Issue 8 Pages 669-675
Terrence P. Tougas, Mark J. Debenedetto, James M. Demott Jr.

Abstract: An oxide surface was formed on a 2-mm Ag-wire electrode by conditioning in 0.1 M NaOH at +0.7 V for 30 min and then at 0.45 V vs. the SCE. The properties of the electrode were studied and it was applied to the electrochemical detection of simple sugars separated by HPLC on a Waters carbohydrate analysis column (30 cm x 3.9 mm) with mobile phase (1 ml/min) of acetonitrile/phosphate buffer of pH 8 (36:11) and by FIA. Calibration graphs were rectilinear for 1-100 µM-galactose, glucose, ribose and xylose.
Electrode Electrochemical analysis HPLC Post-column derivatization

"Analysis Of Pentoses In Dry Wine By High Performance Liquid Chromatography With Post-column Derivatization"
Am. J. Enol. Vitic. 1986 Volume 37, Issue 4 Pages 269-274
Bruce D. Franta, Leonard R. Mattick, and John W. Sherbon

Abstract: Wine samples were adjusted to pH 8 to 9 with concentrated aqueous NH3 and diluted with water before separation of the pentoses on an HPX-87P lead cation-exchange column (30 cm x 7.8 mm), at 70°C, fitted with an Aminex HPX-87C carbohydrate pre-column. Post-column derivatization with tetrazolium blue(I) was achieved with a reagent solution containing 80% ethanol - 0.01 M NaOH - 0.1% I at 1.1 mL min-1 and a 1.8-m reaction coil at 85°; detection was at 520 nm. The rectilinear ranges for xylose, arabinose, ribose, glucose and rhamnose extended up to 2.4 µg (that for fructose up to 1.2 µg), coefficient of variation were ~2% and detection limits were <0.3 µg. The method was only applicable to dry wines as large glucose concentration. (e.g., in sweet wines) affected the determination of xylose.
Wine HPLC Spectrophotometry Heated reaction Post-column derivatization

"Flow Stream Detectors Based On Electrocatalytic Oxidation Of Polyhydroxy Compounds At Silver Oxide Electrodes"
Contemp. Electroanal. Chem. 1990 Volume 1, Issue 1 Pages 275-296
Terrence P. Tougas, Edwin G. E. Jahngen, Michael Swartz

Abstract: Many simple carbohydrates and other polyhydroxy compounds can be oxidized at a silver oxide surface. The oxidation is via an electrocatalytic mechanism involving a Ag(I) oxide. This forms the basis of a flow stream detector operated in an amperometric mode which may be used for either flow injection or high performance liquid chromatography (HPLC) applications. The title electrode has been applied to the detection of simple carbohydrates, triglycerides and nucleic acid components.
Electrode Amperometry Detector