University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Peroxide ion

  • InChI: InChI=1S/O2/c1-2/q-2

@ ChemSpider@ NIST@ PubChem

Citations 6

"Direct Electrocatalytic Determination Of Dissolved Peroxidases"
Anal. Chim. Acta 1996 Volume 329, Issue 3 Pages 231-237
Ulla Wollenberger*, Alma Drungiliene, Walter Stöcklein, Juozas J. Kulys and Frieder W. Scheller

Abstract: 1-(NN-dimethylamine)-4-(4-morpholine)benzene (1-15 mg; I) was dissolved in 200 mg paraffin oil, mixed with 400 mg graphite powder and the mix packed into glass tubes (2.5 cm x 0.85 mm i.d.) and contacted via a Cu wire. Dissolved peroxidases as horseradish peroxidase and microperoxidase II were determined in solutions of 100 mM KCl/H2O2 and containing phosphate or borate buffer at -50 mV vs. Ag/AgCl. In the presence of 66 mM Soerensen phosphate buffer of pH 6.5 and containing 0.4 mM H2O2, calibration graphs were linear for 0.25-100 nM-horseradish peroxidase with a detection limit of 250 pM. Microperoxidase II was determined by FIA using an injection of 100 µL portions of enzyme solution into a carrier stream (0.7 ml/min) of 50 mM borate buffer of pH 9 and detection as above. Calibration graphs were linear up to 500 nM-microperoxidase II with a detection limit of 10 nM.
Amperometry

"Automated Determination Of Peroxides In Olive Oil By Flow Injection"
Analyst 1993 Volume 118, Issue 7 Pages 891-893
J. A. García-Mesa, M. D. Luque de Castro and M. Valcárcel

Abstract: A method for the determination of the peroxide value of olive oil based on flow injection principles is proposed. The method requires no pre-treatment of samples that have undergone natural or forced oxidation as the sensitivity can be accommodated over wide margins (determination range 3.4-537 meq. kg-1). The precision of the method, expressed as relative standard deviation, ranges from 0.7 to 2.4% and the sampling frequency is 15 h-1. A FIA system for the cited determination was developed (manifold illustrated). Sample (1.0-1.5 g) was injected directly into a hexane carrier stream (0.83 ml/min) and diluted by splitting the stream. After merging with a stream of acetic acid (0.54 ml/min), followed by a stream of 0.5% NaI in isopropyl alcohol (0.42 ml/min), the stream was passed through a flow cell connected to a diode-array spectrophotometer for measurement of the generated iodine at 360, 400 or 420 nm for the determination of peroxides in the ranges 3.4-62.0, 16.2-234 or 51.4-537 meq./kg, respectively. The RSD was 0.7-2.4% and the sampling frequency was 15/h.
Oil

"Enzymic Determination Of Peroxides In Non-aqueous Media"
Analyst 1997 Volume 122, Issue 12 Pages 1543-1547
Gerardo Piñeiro Avila, Amparo Salvador and Miguel de la Guardia

Abstract: A fast enzymatic flow injection procedure was developed for the determination of peroxides in non-aqueous samples. The biochemical reaction is effected in a flow injection system using a non-covalently immobilized horseradish peroxidase reactor, followed by spectrophotometric monitoring of p-anisidine. The method provides a limit detection of 0.9 M for hydrogen peroxide, 2.6 M for tert-butyl hydroperoxide and 2.0 M for benzoyl peroxide with a maximum sampling frequency of 60 h-1. The enzyme reactor exhibits enhanced stability in water-saturated toluene, being stable for more than 4 months, and during this period an average number of 250 reactions can be performed with 1 mg of enzyme (220 purpurogallin units). The method permits the determination of peroxide in olive oil and margarine samples without any chemical pre-treatment or extraction of the sample. An average recovery of 98% was found for the determination of hydrogen peroxide in 12 different types of olive oil samples spiked with known amounts of H2O2, indicating the applicability of the procedure to real sample analysis. The procedure was also applied to the determination of H2O2 in four olive oils and a margarine sample and the results were comparable to those obtained by the reference method.
Food Food Spectrophotometry Enzyme

"Determination Of Peroxide Value In Vegetable Oils By An Organic-phase Enzyme Electrode"
Anal. Lett. 1994 Volume 27, Issue 2 Pages 299-308
Mannino, S.;Cosio, M.S.;Wang, J.

Abstract: Vegetable oil (50 µL) was injected without pre-treatment into a carrier solution of CHCl3 (0.7 ml/min) saturated with aqueous 0.1 M phosphate buffer and containing 2 mM ferrocene as mediator and 0.1 M tetrabutylammonium bromide for FIA. Detection was at an enzymatic electrode operated at -0.1 V vs. an undefined reference electrode. The enzymatic electrode was prepared by coating a rough vitreous-carbon electrode with a horse-radish peroxidase/Eastman AQ-55D ester-sulfonic acid polymer mixture applied as solution and dried in situ. The calibration graph was linear from 5-200 ppm of lauroyl peroxide with test recoveries of 98-101%. The RSD (n = 7) were 2.8 and 3.1% for 10 and 52 ppm, respectively.
Oil Electrode

"Electrocatalytic Reduction And Flow Injection Analysis Of Organic Peroxides At Polymeric Tetra-amino Iron Phthalocyanine Modified Electrode"
Anal. Lett. 1996 Volume 29, Issue 9 Pages 1575-1587
Jim Wang

Abstract: Tetra-amino iron phthalocyanine was polymerized on a vitreous C electrode by electropolymerization of the monomer. The electrode was used to develop an FIA method for the determination of organic peroxides using a mobile phase of phosphate buffer of pH 3.2, ionic strength of 0.2 M (1 ml/min) and detection at +0.35 V (vs. SCE). Detection limits were 2 µM-t-butyl-hydroperoxide and 0.7 µM-peracetic acid. The calibration graph was linear from 2.5 µM to 1 mM peracetic acid. The RSD was 2.3%.
Voltammetry Electrode

"Advances In Flow Injection Chemiluminescence Detection"
Fenxi Huaxue 1997 Volume 25, Issue 2 Pages 222-230
Zhou, Y.X.;Zhu, G.Y.

Abstract: A review is presented covering the years 1992 to 1996, with consideration of chemiluminescence and bioluminescence reactions caused by peroxides, phenols, gasification, electrochemistry, biochemistry, etc. along with the developments and application of luminescence detection for FIA. 124 references
Chemiluminescence Bioluminescence Review