Abstract: Use of the chemiluminescent reaction of lucigenin for the determination of trace metal ions and various organic reductants in aqueous media is subject to interference by precipitation of N-methylacridin-9-one in the flow system and observation cell. Sodium dodecyl sulfate (anionic surfactant), hexadecylpyridinium chloride (cationic surfactant) and Triton X-100 (non-ionic surfactant) have been studied as means of solubilizing the reaction product while retaining chemiluminescent emission. Both stopped-flow and continuous-flow systems were studied, with use of the same observation cell (~240 µL), photomultiplier tube biased at 900 V and amplifier. As a result, 0.15 mM Na dodecyl sulfate has been found the most suitable and adopted for use in lucigenin chemiluminescence methods.