University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Oligopeptides

@ ChemSpider@ NIST@ PubChem

Citations 3

"Electrochemical Detection Of Oligopeptides Through The Pre-column Formation Of Biuret Complexes"
J. Chromatogr. A 1991 Volume 542, Issue 2 Pages 345-350
Hweiyan Tsai and Stephen G. Weber

Abstract: The post-column reaction for the formation of electroactive Cu(II) - peptide complexes from larger (>6 amino-acids) peptides requires relatively high temperature and a long reaction time. As an alternative, the incubation of three larger peptides (bradykinin, Tyr8-bradykinin and insulin A chain) with biuret reagent for 20 min at 60°C yields complexes which can be analyzed by flow injection analysis or LC using acidic or basic mobile phases and electrochemical detection. The sensitivity of the latter is around 1.0 nC pmol-1 at 0.80 V and a flow rate of 1 mL min-1. The influence of the column packing material on the electrochemical detector response of the Cu - peptide complexes is also considered.
LC Electrochemical analysis Heated reaction

"Electrogenerated Chemiluminescence Detector For Flow Injection Analysis"
Anal. Sci. 1986 Volume 2, Issue 6 Pages 529-533
M. SATO and T. YAMADA

Abstract: A flow-through electrolytic cell was modified to allow measurement of electrogenerated chemiluminescence (ECL). The quartz optical window was set in front of the platinum plate working electrode. The observed luminescence intensity was amplified by using a lock-in amplification technique. The system was tested by using luminol, which exhibits strong ECL. Samples injected into a carrier solution of 0.1 M Na2CO3 gave luminescence peaks. Non-luminescent compounds could be detected by pre-labelling with luminol. The technique was applied to oligopeptides and bovine serum albumin. The method has the advantage of determining luminescence intensity and electrolytic current simultaneously.
Cow Serum Chemiluminescence Electrode Detector

"Continuous-flow-probe Method For Online Introduction Of Liquid Samples For Detection By Laser Desorption With Resonant Two-photon Ionization In Supersonic Beam Mass Spectrometry"
Rev. Sci. Instrum. 1991 Volume 62, Issue 4 Pages 957-962
David A. Lustig and David M. Lubman

Abstract: Liquid sample was injected into a time-of-flight (TOF) MS instrument via a continuous-flow probe (diagram given) which was coupled to a laser for rapid vaporization and entrainment of the sample into a supersonic jet expansion. The sample was then analyzed by resonance enhanced multiphoton ionization in the TOF device. The method was applied in the analysis of thermally labile biological samples, e.g., neurotransmitters and oligopeptides with quantitation over three orders of magnitude and sensitivity limits in the low ng level.
Biological Mass spectrometry Laser