University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Estrone

  • IUPAC Name: (8R,9S,13S,14S)-3-hydroxy-13-methyl-7,8,9,11,12,14,15,16-octahydro-6H-cyclopenta[a]phenanthren-17-one
  • Molecular Formula: C18H22O2
  • CAS Registry Number: 53-16-7
  • InChI: InChI=1S/C18H22O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-16,19H,2,4,6-9H2,1H3/t14-,15-,16+,18+/m1/s1
  • InChI Key: DNXHEGUUPJUMQT-CBZIJGRNSA-N

@ ChemSpider@ NIST@ PubChem

Citations 3

"Measuring Estrogens Using Flow Injection Immunoanalysis With Liposome Amplification"
Talanta 1993 Volume 40, Issue 12 Pages 1899-1904
Laurie Locascio-Brown* and Steven J. Choquette,

Abstract: A solid-phase competitive immunoassay is performed in flow injection analysis for the measurement of the hormone 17-β-estradiol. The flow injection analysis system incorporates a column-type reactor packed with solid silica particles onto which we have covalently immobilized the antigen 17-β-estradiol. Anti-estradiol is noncovalently conjugated to the liposome through a streptavidin-biotin linkage. When mixed with a sample containing the antigen, the antibody binding sites on the liposomes are complexed which reduces the binding of liposomes to the solid support in a concentration-dependent manner. Sequential immunoassays are performed on-column following a simple regeneration step.
Immunoassay Liposomes

"Microcomputer Data-acquisition And Cumulative Reporting System For Oestrogen And Creatinine Continuous-flow Analysis"
J. Autom. Methods Manag. Chem. 1983 Volume 5, Issue 1 Pages 14-17
D. KEATING, G. J. DUNLOP, A. L. EVANS, R. A. GOWDIE, N. L. GREGORY, T. LEE, and L. G. S. RAO

Abstract: It has been shown that fetal death can be predicted by measuring oestrogen/creatinine ratios on early morning samples of urine and monitoring the trend in results over the last three months of a pregnancy [1]. Bellshill Maternity Hospital serves the Lanarkshire area, which has a birth rate of 10 000 per year. The urinary oestrogen/creatinine ratio test provides inexpensive antenatal monitoring for all patients in the area. The analysis, calculations and cumulative reporting of a test on this scale is both tedious and time-consuming. The introduction of a microcomputer-based system has allowed the full use of this service in Lanarkshire and oestrogen/creatinine analysis can now be carried out on up to 1000 patient samples per week. Only one technician is required for operation and data acquisition, calculations and printing of cumulative results with graphs are performed by the microcomputer system. Disc storage is available for up to 2000 patients. No commercial instrument is yet available to perform and automate these tests.
Computer

"Simultaneous Automated Continuous-flow Analysis Of Total Oestrogen And Creatinine In Pregnancy Urine"
Med. Lab. Sci. 1979 Volume 36, Issue 3 Pages 293-296
Phillips, Susan D.

Abstract: Modifications of the methods of M. Lever et al. (1973) for estrogen and A. L. Chasson et al. (1961) for creatinine (I) are described. Estrogen standards were prepared by diluting the stock solution with urine from males; I standards were diluted with water. The diluted samples for I determination were taken from the estrogen waste-line and further diluted with water containing Brij-35 (30%) before reacting with alkaline picrate. This dilution decreases the need for dialysis of the urine and also decreases interferences by other substance. Any urines showing +++ or greater for glucose and(or) ketones, as determined by the Keto-diastix, were treated with octan-2-ol and NaBH4 (in glucose presence) and by boiling (in ketones presence). The through time for this automated determination is 11 min for I and 18 min for estrogens. (SFS)
Urine Clinical analysis Interferences