Contact Info
Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf
Equilenin sulfate
- InChI: InChI=1S/C19H20O5S.Na/c1-19-9-8-15-14-4-2-12(11-24-25(21, 22)23)10-13(14)3-5-16(15)17(19)6-7-18(19)20;/h2-5,10,17H,6-9,11H2,1H3, (H,21,22,23);/q;+1/p-1/t17-,19-;/m0./s1
Citations 1
"Comparison Of Capillary Gas-chromatographic Method And Automated Spectrophotofluorimetric Methods For Measuring Content Uniformity Of Conjugated Oestrogens In Pharmaceutical Preparations"
J. Autom. Methods Manag. Chem.
1991 Volume 13, Issue 3 Pages 115-117
J. D. RUSSELL, J. MUMLEY, M. SPRING, A. KUTZ, and R. N. JOHNSON
Abstract:
Conjugated oestrogens were extracted from tablets with water, reacted with 0.15% dicyclohexylamine acetate solution in 1,1,2-trichloroethane and extracted into acetic acid - methanol - water (1:1:2) in a flow injection system (Technicon Inc., New York). The complex formed was extracted into hot aqueous 70% H2SO4 and detected at 525 nm (excitation at 415 nm). The coefficient of variation (n = 10) was 2.6%; results compared well with those obtained by GC. Sample throughput was 20 h-1. The cited method has been approved by the FDA.
Pharmaceutical
GC
Fluorescence
Sample preparation
Automation
Extraction
Standard method