University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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l-Methionine

  • IUPAC Name: (2S)-2-amino-4-methylsulfanylbutanoic acid
  • Molecular Formula: C5H11NO2S
  • CAS Registry Number: 63-68-3
  • InChI: InChI=1S/C5H11NO2S/c1-9-3-2-4(6)5(7)8/h4H,2-3,6H2,1H3,(H,7,8)/t4-/m0/s1
  • InChI Key: FFEARJCKVFRZRR-BYPYZUCNSA-N

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Citations 3

"Flow Reversal And Flow Recycle Air-segmented Flow Injection For Simultaneous Determination Of A Binary Mixture"
Anal. Chim. Acta 1995 Volume 304, Issue 3 Pages 333-337
Yunsheng Hsieh and S. R. Crouch*

Abstract: Computer-controlled flow reversal and recycle techniques were combined with an air-segmented flow injection system for the simultaneous kinetic analysis of binary mixtures of amino-acids. The method was applied to mixtures of L-phenylalanine (I) and L-methionine (II) using the L-amino-acid oxidase/Trinder reaction. The apparatus allowed a mixture of the analytes and enzyme reagent to by pass through the spectrophotometric detector cell several times. The calibration graphs were linear for 0.05-0.2 mM I and 0.1-0.5 mM II. The results for the analysis of mixtures containing up to 0.45 mM of each analyte were presented. The relative errors ranged from -8 to 36%. The lower dispersion of the proposed method resulted in an improvement over normal flow-reversal flow injection systems.
Spectrophotometry Air segmentation Flow reversal Kinetic

"Determination Of Enzyme Substrates With An Extended Range Of Linearity"
Anal. Chim. Acta 1995 Volume 309, Issue 1-3 Pages 251-258
Yun-Sheng Hsieh and S. R. Crouch*

Abstract: Portions of 1 mL of L-methionine, L-phenylalanine and L-tryptophan were mixed with 1 mL of a solution comprising 5 mL 10.7 mM 3,5-dichloro-2-hydroxyphenyl sulfonic acid, 10 mM KCl, 10 mg L-amino-acid oxidase, 16 mg horseradish peroxidase and 40 mL 100 mM phosphate buffer (pH not given). The oxidative deamination of the L-amino-acids was monitored at 510 nm and the data modelled to a one-compartment kinetic model using a commercial non-linear statistical software package. Similar reactions were performed using air-segmented continuous and stopped-flow analysis as described previously (Ibid., 1994, 296, 333). The accuracy and precision of the model were not significantly different with 95% confidence in comparison with the Michaelis-Menten model and double-reciprocal plots.
Spectrophotometry Stopped-flow Method comparison

"Electrocatalysis With A Dirhodium-substituted Polyoxometalate Anchored In A Xerogel-based Composite. Application To The Oxidation Of Methionine And Cystine At Physiological PH"
Electroanalysis 1998 Volume 10, Issue 18 Pages 1237-1240
Mark E. Tess, James A. Cox

Abstract: Replacing a tungsten oxide group of phosphotungstic acid with a Rh(II) acetate dimer yielded a species, Rh2POM, that catalyzed the electrochemical oxidation of L-methionine (L-Met), L-cystine, and As(III). The electrocatalytic activity of Rh2POM was greater than that of the simple Rh(II) acetate dimer in homogeneous solution A conducting carbon-composite electrode (CCE) comprising Rh2POM and carbon powder in a SiO2 matrix prepared by sol-gel chemical was catalytically active over the pH range 2-10. The CCEs produced voltammetric responses toward L-Met and L-cystine that were pH independent over the above range and were stable for at least 4 mo under dry storage. If passivated, the catalytic activity was generated by polishing. For example, a RSD of 2.9% (5 points) was obtained when the surface was polished between cyclic voltammetric experiments on 5.0 mM L-Met at pH 7.4. A least squares fit of flow injection amperometric data obtained over the range 5-156 µM L-Met (7 points) yielded the following: slope 0.85 µA mM 1; intercept 0.05 µA; and r = 0.995. The detection limit was 2.3 µM.
Amperometry Electrode Sensor pH Apparatus Detector Catalysis