University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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d-Mannitol

  • IUPAC Name: (2R,3R,4R,5R)-hexane-1,2,3,4,5,6-hexol
  • Molecular Formula: C6H14O6
  • CAS Registry Number: 69-65-8
  • InChI: InChI=1S/C6H14O6/c7-1-3(9)5(11)6(12)4(10)2-8/h3-12H,1-2H2
  • InChI Key: FBPFZTCFMRRESA-KVTDHHQDSA-N

@ ChemSpider@ NIST@ PubChem

Citations 4

"Flow Injection Determination Of D-mannitol With Immobilized Mannitol Dehydrogenase"
Anal. Chim. Acta 1991 Volume 244, Issue 1 Pages 105-107
Nobutoshi Kiba, Yukari Inoue and Motohisa Furusawa

Abstract: In the flow injection method described (diagram of apparatus given), 5 mM NAD+ was mixed with 0.2 M carbonate buffer of pH 10.0 (total flow-rate 0.5 mL min-1) and passed through a mixing coil (1 m x 0.5 mm). Sample solution (50 µL) was injected into the stream, the mixture was passed through a column reactor (5 cm x 4 mm) containing mannitol dehydrogenase immobilized on poly(vinyl alcohol) beads (cf., Ibid., 1991, 243, 183) at 37°C and the NADH produced was detected fluorimetrically at 465 nm (excitation at 340 nm). The calibration graph was rectilinear for 0.5 to 100 µM-D-mannitol and the detection limit was 0.1 µM. The sampling rate was 30 h-1 and the coefficient of variation (n = 12) was 1%. The method was applied in the analysis of celery juice and chewing gum (prep. described); results compared well with those by a chromatographic method. The method should also be applicable to biological samples.
Juice Food Fluorescence Buffer Column Immobilized enzyme

"Nickel Oxide Dispersed In A Graphite/poly(vinyl Chloride) Composite Matrix For An Electrocatalytic Amperometric Sensor For Alditols In Flow Injection Analysis"
Anal. Chim. Acta 1995 Volume 307, Issue 1 Pages 43-48
Tommaso R. I. Cataldi* and Diego Centonze

Abstract: A chemically modified electrode was prepared with 50% NiO, 7% PVC and graphite and packing the mixture into a PTFE cavity (4 mm i.d.). The electrode was used in a FIA system for the amperometric detection of alditols. The wall-jet detection cell also had a Ag/AgCl(3 M KCl) reference electrode and a stainless-steel auxiliary electrode; the working electrode was maintained at +0.5 V. The carrier electrolyte (0.5 ml/min) was 0.1 M NaOH and the sample injection volume was 50 µL. Calibration graphs were linear up to 50 mM alditol (or 25 mM sorbitol) and detection limits were 0.02 M for sorbitol, myo-inositol and xylitol, 0.05 M for dulcitol, 0.08 M for mannitol and 0.1 M for D-arabitol. The RSD for 0.5 mM of analyte were 0.8-1.5% (n = 4-6). The performance of the electrode was stable over 72 h.
Amperometry Electrode Electrode Sensor Catalysis Flowcell

"Detection Of Polyols And Sugars By Cuprammonium Ion In The Presence Of Strong Base"
Anal. Biochem. 1987 Volume 165, Issue 2 Pages 392-398
D. B. McKay, G. P. Tanner, D. J. Maclean* and K. J. Scott

Abstract: The cuprammonium reagent of Grimble et al. (Ibid., 1983, 128, 422) was modified so that final concentration. of active constituents in the reaction mixture were: 1.0 mM Cu(II), 0.4 M NH3 and 0.2 M NaOH (in excess); the absorbance (at 280 nm) of the modified reagent blank was <50% of that of previous methods. The new reagent was most sensitive for the colorimetric determination of polyols, especially D-glucitol, but it also produced a better color yield for some aldoses and disaccharides than the original reagent. It was used for post-column HPLC detection of various sugars and polyols; calibration graphs for D-glucitol, D-mannitol and D-glucose were rectilinear from 40 to 180 nmol, 0 to 150 nmol and 0 to 300 nmol, respectively. The method was applied in analysis of fungal mycelia.
Fungus HPLC Spectrophotometry Post-column derivatization

"Anion-exchange Chromatography With Electrochemical Detection Of Alditols And Sugars At A Cu2o-carbon Composite Electrode"
J. Chromatogr. A 1997 Volume 773, Issue 1-2 Pages 115-121
Tommaso R. I. Cataldia,*, Diego Centonzea, Innocenzo G. Casellaa and Elio Desimonib

Abstract: An anion-exchange column coupled with an amperometric sensor was used for the quantitative analysis of alditols and simple sugars. The sensing electrode is composed of cuprous oxide dispersed in a graphite powder-polyethylene composite matrix. The resulting Cu2O-carbon composite electrode is stable in alkaline media and possesses good sensitivity, wide linear dynamic ranges and low detection limits for alditols, mono- and disaccharides. Alditols and carbohydrates are weakly ionizable compounds, so an anion-exchange column was employed for their chromatographic separation with an alkaline eluent. The separation problems due to the presence of low but uncontrolled amounts of carbonate in the alkaline mobile phase have been largely solved by the addition of Ca2+ or Ba2+ at a millimolar level and the consequent formation of carbonate insoluble salts. Using this strategy, the alkaline eluent provides improved separations without compromising the column's lifetime, electrode performance and chromatographic system. Under the optimal operating conditions, the detection limits of D-sorbitol, D-mannitol and D-glucose were 50, 40 and 80 pmol, respectively, with a linear concentration range up to 5 mM. Examples of applications, which include the separation and detection of D-sorbitol, D-mannitol and common sugars present in food samples, are illustrated. 35 References
Food Amperometry Electrode Electrode Ion exchange Optimization