Abstract: A sample solution was diluted with water to 25 mL and then analyzed by FIA (diagram given). The sample mixed with streams of 0.05 M KMnO4 and 1 M H6P4O13 in the flow injection system and the chemiluminescence thus generated was measured. The calibration graph for levodopa (dopa) was linear from 0.4-80 mg/l and the detection limit was 62 g/l. The method was also used to analyze tablets; RSD were 1.6%. The results were compared with those obtained by UV spectrophotometry. None of the investigated co-existing components interfered. The FIA sample throughput was 120/h. In this paper,a new chemiluminescence system of potassium permanganate-levodopa was investigated and a new chemiluminescence method for the determination of levodopa with flow injection technique was established. The concentration range of linear response is 4 x 10^-4~8 x 10^-2g/L.The detection limit is 6.2 x 10^-5g/L with a relative standard deviation of 1.6% for the determination of 4 x 10^-3g/L of levodopa(n=11). The method has been used to determine the content of levodopa in tablets with satisfactory results.