University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Immunoglobulin G, mouse

@ ChemSpider@ NIST@ PubChem

Citations 4

"A Disposable Protein A-based Immunosensor For Flow Injection Assay With Electrochemical Detection"
Anal. Chim. Acta 2000 Volume 404, Issue 2 Pages 187-194
Christophe Valat, Benoit Limoges, Denis Huet and Jean-Louis Romette

Abstract: Low-cost disposable immunosensors were produced by covalent binding of Protein A or G on graphite-polystyrene screen-printed electrodes, and they were used in a fully automated flow injection analysis (FIA) system, allowing the kinetics of IgG binding to Protein A or G to be improved by forced convection. The displacement of rabbit IgG bound to Protein A or G by mouse IgG isotypes (IgG(1) or IgG(2a)) was studied. A FIA immunoassay of mouse IgG(2a) was performed at a Protein A-based immunosensor with a good sensibility (down to 0.02 µg ml-1) and a total assay time of 19 min. It was shown that the immunosensor combines the advantages of being reusable for more than 30 assay cycles in flow injection analysis, and disposable when necessary.
Potentiometry Electrode Electrode Immunoassay Sensor Apparatus Immobilized protein

"A Flow Injection Continuous Filtration Approach For The Automatic Determination Of Monoclonal Antibodies"
Anal. Chim. Acta 1996 Volume 331, Issue 3 Pages 245-251
J. M. Fernández-Romero and M. D. Luque de Castro*

Abstract: A FIA system for heterogeneous microassay was evaluated by determining monoclonal mouse IgG using an anti-mouse IgG - peroxidase conjugate and fluorimetric detection. Portions (100 µL) of IgG solution and anti-IgG - peroxidase conjugate solution were injected simultaneously into two carrier streams (both 0.8 ml/min) which were then merged to allow the the immuno-precipitate to form. This was filtered off using an inline filter. The conjugate passed was merged with a reagent stream containing 100 mM NH4Cl, 3 mM 4-hydroxyphenylacetic acid, 5 mM H2O2 and 5 mM NaOH. The reaction product (4-hydroxyphenylacetic acid dimer) was detected by fluorimetry at 425 nm (excitation at 315 nm). The dynamic linear range for IgG was 1-200 ng/ml, the detection liwas 0.1 ng/ml and the RSD (n = 33) was 3% for 10 ng/ml IgG.
Fluorescence Filtration Automation

"Real-time Monitoring Of Immunochemical Interactions With A Tantalum Capacitance Flow-through Cell"
Anal. Chem. 1992 Volume 64, Issue 9 Pages 997-1003
Andreas Gebbert, Manuel Alvarez-Icaza, Walter Stoecklein, and Rolf D. Schmid

Abstract: A flow-through cell for the real-time capacitance monitoring of immunochemical interactions has been developed. It consists of a tantalum strip onto which tantalum oxide was grown electrochemically to a layer thickness as small as 5 nm. Antibody or antigen was immobilized onto the tantalum oxide surface, and binding of the corresponding analyte resulted in modification of the elec. capacitance of the system. With mouse IgG as the ligand, real-time monitoring of anti-mouse-IgG in the nanogram per mL range was possible. The behavior of the system with respect to analyte size and concentration. was investigated. The tantalum/tantalum oxide/electrolyte system can be manufactured easily and reproducibly.
Capacitance Flowcell Process monitoring Immobilized antibody Immobilized antigen

"Highly Sensitive Determination Of Hydrogen Peroxide And Peroxidase With Tetrathiafulvalene-based Electrodes And The Application In Immunosensing"
Biosens. Bioelectron. 1997 Volume 12, Issue 1 Pages 43-52
Frank Wendzinski, Bernd Gründig, Reinhard Renneberg and Friedrich Spener*

Abstract: A tetrathiafulvalene modified graphite electrode coupled with horseradish peroxidase is described, capable of monitoring H2O2 by FIA with a detection limit of 7 nM at a potential of +20 mV vs. a SCE. Holding the H2O2 constant, the detection limit of horseradish peroxidase was 150 pM. The system was evaluated and applied to the determination of 5-100 ng/ml of rabbit IgG as a model analyte by sandwich immunoassay.
Biological Immunoassay Electrode Electrode