Contact Info
Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf
Imipenem, N-Formimidoyl thienamycin
- IUPAC Name: (5R,6S)-3-[2-(aminomethylideneamino)ethylsulfanyl]-6-[(1R)-1-hydroxyethyl]-7-oxo-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylic acid
- Molecular Formula: C12H17N3O4S
- CAS Registry Number: 64221-86-9
- InChI: InChI=1S/C12H17N3O4S/c1-6(16)9-7-4-8(20-3-2-14-5-13)10(12(18)19)15(7)11(9)17/h5-7,9,16H,2-4H2,1H3,(H2,13,14)(H,18,19)/t6-,7-,9-/m1/s1
- InChI Key: ZSKVGTPCRGIANV-ZXFLCMHBSA-N
Citations 1
"Quantification Of Imipenem's Primary Metabolite In Plasma By Post-column Chemical Rearrangement And UV Detection"
Pharm. Res.
1991 Volume 8, Issue 1 Pages 33-39
Donald G. Musson, Richard Hajdu, William F. Bayne and John D. Rogers
Abstract:
Pre-treated samples (prep. described) were subjected to HPLC on a column (10 cm x 8 mm) of Resolve C18 Radial-PAK equipped with a column of RCSS Guard PAK C18 with a mobile phase of tetrabutylammonium hydrogen sulfate - H3PO4 - water, adjusted to pH 6.85 with 1 M KOH. The eluate was diluted with 0.426 M H3PO4 (0.6 mL min-1, passed through a column (25 cm x 4.6 mm) packed with 40 µm glass beads and the absorbance was measured at 295 and 320 nm. Calibration graphs were rectilinear for 1 to 100 µg mL-1 of the cited metabolite (I) in plasma and dialysate and for 5 to 100 µg mL-1 in urine . Coefficients of variation were 10%.
Blood Plasma
Urine
HPLC
Spectrophotometry
Column
Glass beads
pH
Post-column derivatization