Abstract: A 20 µL portion of amine was injected into a stream of aqueous 90% acetonitrile containing 0.02 M H2O2. The mixture was passed at 1.5 ml/min to a flow cell together with 0.5 mM bis-[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl] oxalate and 0.1 µM-Sulforhodamine 101 in acetonitrile. The chemiluminescence was monitored by a photomultiplier tube. The detection limits of 55 amines were studied without derivatization; 28 were detected at levels of 0.1 nM to 4 µM, with linear calibration graphs up to 0.1 to 1 mM, respectively. The RSD for 1 µM-triethylamine was 4.8% (n = 6). The method was selective and sensitive for aliphatic amines and imidazoles, and the detection limits were lower than those obtained using derivatization-fluorimetry methods. The technique was applied to the determination of histamine in fish. The recovery was 94-96.2% and the results were comparable with those measured by a LC method using fluorescence detection after derivatization.