University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Hydrocortisone

  • IUPAC Name: (8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-3-one
  • Molecular Formula: C21H30O5
  • CAS Registry Number: 50-23-7
  • InChI: InChI=1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
  • InChI Key: JYGXADMDTFJGBT-VWUMJDOOSA-N

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Citations 2

"Flow Injection Chemiluminometric Analysis Of Some Steroids By Their Sensitizing Effect On The Bromate-sulfite Reaction"
Anal. Chim. Acta 1990 Volume 239, Issue 2 Pages 195-202
Aristotelis B. Syropoulos, Evangelos G. Sarantonis and Antony C. Calorkerinos

Abstract: The solution of cortisone (I), hydrocortisone, corticosterone, testosterone (II) or progesterone (III) were injected into 2 mM NaBrO3 in 20 mM H2SO4 at 1.5 mL min-1. After mixing with 0.5 mM SO32- the luminescence was measured with a photomultiplier. The responses were rectilinear between 0.5 and 5 to 6 µg mL-1 except for I which was from 0.5 to 20.0 µg mL-1, with limits of detection between 0.1 and 0.4 µg mL-1. The coefficient of variation (n = 6) for 0.5 and 4.0 µg mL-1 of III were 2.3 and 0.5%, respectively, which were typical for all the steroids. Recoveries of II from various excipients were between 95.8 and 104.0%.
Chemiluminescence Sensitivity Detection limit Indirect

"Reductive Electrochemical Detection Of 3-oxosteroids In Non-aqueous Solvents"
J. Pharm. Pharmacol. 1987 Volume 39, Issue Suppl. Pages 47P-NA
Pryce Jones, R.H.;Carr, B.E.;Hossein, A.M.

Abstract: Cortisone, hydrocortisone, prednisone, prednisolone, dexamethasone and betamethasone could be separated by HPLC on a 15-cm Hypersil (5 µm) column with methanol - 1,2-dichloroethane (3:197) as mobile phase (1.0 mL min-1). Each compound gave a response in a BAS LC4A electrochemical system, incorporating a mercury-coated gold electrode at -1.75 V, when 0.1 M tetrabutylammonium tetrafluoroborate (I) was added to the solvent as carrier electrolyte; calibration graphs were rectilinear and the sensitivity was 200 ng per injection. However, none of the steroids was retained on the column in the presence of I. The method should be applicable in flow injection analysis, or with post-column addition of electrolyte.
HPLC Electrode