University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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γ-Globulin

@ ChemSpider@ NIST@ PubChem

Citations 8

"Determination Of A Small Amount Of A Biological Constituent By The Use Of Chemiluminescence. 1. The Flow Injection Analysis Of Protein"
Bull. Chem. Soc. Jpn. 1983 Volume 56, Issue 5 Pages 1382-1387
Tadashi Hara,Motohiro Toriyama and Kazuhiko Tsukagoshi

Abstract: A new method in which luminol-hydrogen peroxide luminescent system is used has been proposed for the determination of the presence of protein. Since the catalytic activity of copper(II) for the chemiluminescent reaction between luminol and hydrogen peroxide decreased when copper(II) interacted with polypeptide linkage, this phenomenon was applied to the determination of protein. Determination of protein was carried out by a flow-injection method. The effects of reagent concentration, flow-rate, and reaction time on the analytical value were examined and the conditions for the determination of protein were established. Similar calibration curves were obtained for human serum albumin, bovine serum albumin, bovine serum α-globulin, and bovine serum γ-globulin. According to the present flow-injection method using chemiluminescent reaction, a small amount of protein could be conveniently and economically determined over a wide range of concentration, 7 x 10^-4 - 7 x 10^-2 g dm-;3, with the detection limit of 0.2 µg and at the rate of about 30 samples per hour. The present method was applicable to the determination of protein in serum.
Serum Human Cow Serum Clinical analysis Chemiluminescence Catalysis Optimization

"Determination Of A Small Amount Of A Biological Constituent By The Use Of Chemiluminescence. 2. Determination Of Albumin As A Model Protein By Means Of The Flow Injection Analysis Using A Cobalt(III) Complex Compound As A Catalyst"
Bull. Chem. Soc. Jpn. 1984 Volume 57, Issue 1 Pages 289-290
Tadashi Hara,Motohiro Toriyama and Kazuhiko Tsukagoshi

Abstract: A new procedure for the determination of 8 x 10^-9 - 3 x 10^-7 mol L-1 bovine serum albumin as a model protein has been established on the basis of the fact that the catalytic activity of cis-tetraarnminediaquacobalt(III) sulfate for the chemiluminescent reaction between luminol and hydrogen peroxide decreases in the presence of bovine serum albumin.
Blood Serum Serum Human Chemiluminescence Clinical analysis Catalysis Heated reaction

"Determination Of A Small Amount Of A Biological Constituent By The Use Of Chemiluminescence. 3. Flow Injection Analysis Of Protein By Direct Injection"
Bull. Chem. Soc. Jpn. 1984 Volume 57, Issue 6 Pages 1551-1555
Tadashi Hara,Motohiro Toriyama and Kazuhiko Tsukagoshi

Abstract: A flow-injection analysis method of protein by direct injection has been established by use of the luminol-hydrogen peroxide luminescence system. The determination of protein is based on the measurement of the decreasing catalytic activity of copper(II) for the chemiluminescent reaction between luminol and hydrogen peroxide. The present flow-injection analysis in which a protein solution is directly injected is quite different from the previous one in which a protein solution is injected after having been previously allowed to react with copper(II) outside the flow-through system. The optimum conditions For the determination of protein were determined with regard to reagent concentration, flow rate, reaction time, and reaction temperature. The present method is simple, rapid, and applicable to the determination of 2 x 10^-4 - 1 x 10^-1 g L-1 of protein with 40 ng of detection limit at a rate of about 30 samples per hour. The present method was also applied to the determination of the ratio of albumin to globulin in a sample. The present flow-injection system is expected to be a sensitive detector for the determination of small amounts of protein.
Serum Human Chemiluminescence Heated reaction Optimization

"Determination Of A Small Amount Of A Biological Constituent By The Use Of Chemiluminescence. 6. The Flow Injection Analysis Of Protein Using A 1,10-phenanthroline Hydrogen Peroxide System"
Bull. Chem. Soc. Jpn. 1986 Volume 59, Issue 6 Pages 1833-1838
Tadashi Hara,Takashi Ebuchi,Akihiro Arai and Masakatsu Imaki

Abstract: The method is based on the inhibition by protein of the catalysis by Cu(II) of the chemiluminescent reaction between 1,10-phenanthroline and H2O2. Calibration graphs of log. photomultiplier peak area vs. log. protein concentration. were rectilinear for 20 µg L-1 to 0.1 g L-1 of bovine serum albumin(I) and 20 µg L-1 of bovine γ-globulin. The detection limit was 1 ng of I, but this could be improved to 250 pg (with improvement in the limit of determination to 5 µg l-1) by inclusion of arginine in the Cu(II) solution, which represented a fortyfold improvement with respect to that with the luminol - H2O2 reaction as described in Part (IV) (Ibid., 1985, 58, 109). (For Part V see Anal. Abstr., 1986, 48, 1D189).
Cow Serum Chemiluminescence Catalysis

"Determination Of A Small Amount Of A Biological Constituent By The Use Of Chemiluminescence. 11. Determination Of Protein Using A 1,10-phenanthroline - Hydrogen Peroxide - Osmium(VIII) System"
Bull. Chem. Soc. Jpn. 1987 Volume 60, Issue 6 Pages 2031-2035
Tadashi Hara and Kazuhiko Tsukagoshi

Abstract: Formation of a complex between the analyte protein and Os(VIII) present in known excess was used to lessen the amount of Os available to catalyse the formation of chemiluminescence in the 1,10-phenanthroline - H2O2 system. Reagent solution were 0.12 mM 1,10-phenanthroline, 5% H2O2 and 1.3 mM Os(VIII) and the flow injection system was as previously described (Ibid., 1986, 59, 3681). Calibration graphs were established for the determination of bovine and human serum albumins and human serum γ-globulin under optimum conditions. From 5 µg L-1 to 1 mg L-1 of protein could be determined with a detection limit of 0.25 ng and a coefficient of variation of 2.9% at 0.1 mg L-1 (n = 10).
Cow Serum Serum Human Chemiluminescence Optimization

"Determination Of A Small Amount Of A Biological Constituent By The Use Of Chemiluminescence. 15. Zeolite Column - Chemiluminescence Detection System For The Separation And The Determination Of A Protein Mixture"
Bull. Chem. Soc. Jpn. 1989 Volume 62, Issue 5 Pages 1501-1508
Tadashi Hara,Kazuhiko Tsukagoshi and Yuichiro Kurita

Abstract: The adsorption behavior of proteins (human serum albumin and γ-globulin) on molecular sieve 13X (100 to 120 mesh; pore diameter 10 .angstrom.) was examined by the depletion method in aqueous solution and by using a column, with detection at 280 nm in each instance. Globulin was adsorbed by the zeolite but albumin was not. Conditions were optimized for separating and determining the two proteins by a flow injection - chemiluminescence system (illustrated) with 1,10-phenanthroline - H2O2 - Cu(II) (Ibid., 1986, 59, 1833) as reagent. A detection limit of 1 ng, an analytical range of 0.02 to 5 mg L-1 and coefficient of variation of 5.4 and 8.3% (n = 5) at 1 mg L-1 were achieved for albumin and γ-globulin, respectively, by using a microbore column (25 cm x 1 mm) of NaX zeolite, 10 mM phosphate buffer of pH 6.4 to 6.5 as carrier solution, and 13.2 mM NaOH - 5 mM NaCl (pH 12.0) as eluent. The method was applied in analysis of serum.
Blood Serum LC Chemiluminescence Optimization Detection limit Buffer

"Sensitive Determination Of Proteins By FIA With Coulometric Detection"
Bunseki Kagaku 1989 Volume 38, Issue 9 Pages 454-457
Kubo, H.;Huang, Y.S.;Kinoshita, T.;Nakazawa, H.

Abstract: Sample solution (5 ml) was injected into carrier/reagent solution comprising 0.3 M Na2HPO4 - 1 mM CuSO4 - 20 mM NH3 (pH 12) and passed to a reaction coil (5 m x 0.5 mm) operated at 95°C, and then to a cooling coil (1 m x 0.5 mm) operated at 10°C. Coulometric detection was carried out at 0.7 V. Calibration graphs were rectilinear for 10 to 100 ng of bovine serum albumin (I), human serum albumin, human γ-globulins and ovalbumin. The coefficient of variation (n = 10) at 10 and 25 ng of I were 3.5 and 3.1%, respectively. The detection limit for I was 2.5 ng. Histidine, cysteine, tryptophan and tyrosine were sensitively detected.
Cow Serum Serum Human Coulometry Heated reaction

"Investigation Of A Fluorimetric Assay For Proteins By Phthalaldehyde"
Fenxi Huaxue 1987 Volume 15, Issue 1 Pages 17-21
Ding, W.;Xu, S.;Zhang, R.

Abstract: A fluorimetric determination by using phthalaldehyde was developed for proteins with particular emphasis on its use in HPLC as a post-column derivatization procedure. The effect of several factors on the fluorescence intensity of proteins was studied. Sensitivity of the method was <0.15 µg mL-1 which was five- to nine-fold better than values obtained by using UV detection at 230 nm. A rectilinear response was obtained for γ-globin from 0.04 to 2.0 mg mL-1.
HPLC Fluorescence Post-column derivatization