University of North Florida
Browse the Citations
-OR-

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

View Stuart Chalk's profile on LinkedIn

d-Fructose

  • IUPAC Name: (3S,4R,5R)-2-(hydroxymethyl)oxane-2,3,4,5-tetrol
  • Molecular Formula: C6H12O6
  • CAS Registry Number: 6347-01-9
  • InChI: InChI=1S/C6H12O6/c7-1-3(9)5(11)6(12)4(10)2-8/h3,5-9,11-12H,1-2H2/t3-,5-,6-/m1/s1
  • InChI Key: LKDRXBCSQODPBY-VRPWFDPXSA-N

@ ChemSpider@ NIST@ PubChem

Citations 3

"Simultaneous Enzymic And Tautomeric Reactions Of D-fructose In A Reactor With Immobilized Hexokinase"
Anal. Chim. Acta 1993 Volume 271, Issue 1 Pages 39-45
Mikael Skoog and Gillis Johansson*

Abstract: A computer model was developed for the description of the simultaneous enzymatic conversion and tautomerization reactions during phosphorylation of D-fructose by hexokinase in a small immobilized-enzyme reactor. The predicted conversion to fructose-6-phosphate was compared with the value observed in a flow injection system. An iteration procedure was applied to allow the evaluation of competing enzymatic tautomerization rate constants with consideration of four tautomers. The slow ring opening of β-fructopyranose was seen to complicate the determination of fructose by rapid tautomer-selective enzymatic methods.
Immobilized enzyme Reactor Modeling

"Amperometric Mediated Carbon-paste Biosensor Based On D-fructose Dehydrogenase For The Determination Of Fructose In Food Analysis"
Biosens. Bioelectron. 1997 Volume 12, Issue 12 Pages 1233-1243
Pedro A. Paredesa, Josefina Parelladaa, Víctor M. Fernándezc, Ioanis Katakisb and Elena Domíngueza,*

Abstract: A new mediated amperometric biosensor for fructose is described. The sensor is based on a commercially available D-fructose dehydrogenase. The enzyme is incorporated in a carbon paste matrix containing Os(bpy)(2)Cl-2 as redox mediator that achieves electron transfer at 0.1 V (versus Ag/AgCl) with maximum apparent current densities of 1.2 mA/cm(2). The dependence of the steady-state current on the loading of the mediator and the enzyme, other electrode construction parameters, the operating potential, the pH and the temperature was studied. In the steady-state mode the response current was directly proportional to D-fructose concentration from 0.2 to 20 mM with a detection limit of 35 µM (signal-to-noise ratio, S/N, 3). In the flow injection analysis mode the response current was directly proportional to D-fructose concentration from 0.5 to 15 mM with a detection limit of 115 µM (S/N 3). The sensor was used for the determination of fructose in food samples in a flow injection system and validated with a commercial enzyme kit. (C) 1998 Elsevier Science S.A. 32 References
Food Amperometry Sensor Electrode Immobilized enzyme Optimization

"Flow Stream Detectors Based On Electrocatalytic Oxidation Of Polyhydroxy Compounds At Silver Oxide Electrodes"
Contemp. Electroanal. Chem. 1990 Volume 1, Issue 1 Pages 275-296
Terrence P. Tougas, Edwin G. E. Jahngen, Michael Swartz

Abstract: Many simple carbohydrates and other polyhydroxy compounds can be oxidized at a silver oxide surface. The oxidation is via an electrocatalytic mechanism involving a Ag(I) oxide. This forms the basis of a flow stream detector operated in an amperometric mode which may be used for either flow injection or high performance liquid chromatography (HPLC) applications. The title electrode has been applied to the detection of simple carbohydrates, triglycerides and nucleic acid components.
Electrode Amperometry Detector