Contact Info
Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf
Estrone sulfate
- IUPAC Name: [(8R,9S,13S,14S)-13-methyl-17-oxo-7,8,9,11,12,14,15,16-octahydro-6H-cyclopenta[a]phenanthren-3-yl] hydrogen sulfate
- InChI: InChI=1S/C18H22O5S.Na/c1-18-9-8-14-13-5-3-12(23-24(20,21)22)10-11(13)2-4-15(14)16(18)6-7-17(18)19;/h3,5,10,14-16H,2,4,6-9H2,1H3,(H,20,21,22);/q;+1/p-1/t14-,15-,16+,18+;/m1./s1
- InChI Key: JKKFKPJIXZFSSB-CBZIJGRNSA-N
Citations 1
"Comparison Of Capillary Gas-chromatographic Method And Automated Spectrophotofluorimetric Methods For Measuring Content Uniformity Of Conjugated Oestrogens In Pharmaceutical Preparations"
J. Autom. Methods Manag. Chem.
1991 Volume 13, Issue 3 Pages 115-117
J. D. RUSSELL, J. MUMLEY, M. SPRING, A. KUTZ, and R. N. JOHNSON
Abstract:
Conjugated oestrogens were extracted from tablets with water, reacted with 0.15% dicyclohexylamine acetate solution in 1,1,2-trichloroethane and extracted into acetic acid - methanol - water (1:1:2) in a flow injection system (Technicon Inc., New York). The complex formed was extracted into hot aqueous 70% H2SO4 and detected at 525 nm (excitation at 415 nm). The coefficient of variation (n = 10) was 2.6%; results compared well with those obtained by GC. Sample throughput was 20 h-1. The cited method has been approved by the FDA.
Pharmaceutical
GC
Fluorescence
Sample preparation
Automation
Extraction
Standard method