Abstract: The solution of cortisone (I), hydrocortisone, corticosterone, testosterone (II) or progesterone (III) were injected into 2 mM NaBrO3 in 20 mM H2SO4 at 1.5 mL min-1. After mixing with 0.5 mM SO32- the luminescence was measured with a photomultiplier. The responses were rectilinear between 0.5 and 5 to 6 µg mL-1 except for I which was from 0.5 to 20.0 µg mL-1, with limits of detection between 0.1 and 0.4 µg mL-1. The coefficient of variation (n = 6) for 0.5 and 4.0 µg mL-1 of III were 2.3 and 0.5%, respectively, which were typical for all the steroids. Recoveries of II from various excipients were between 95.8 and 104.0%.