University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Cis-platin

  • IUPAC Name: azane;dichloroplatinum(2+)
  • Molecular Formula: Cl2H6N2Pt+2
  • InChI: InChI=1S/2ClH.2H3N.Pt/h2*1H;2*1H3;/q;;;;+2/p-2
  • InChI Key: BSJGASKRWFKGMV-UHFFFAOYSA-L

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Citations 4

"Determination Of Cisplatin And Cis-diammineaquachloroplatinum(II) Ion By Liquid Chromatography Using Post-column Derivatization With Diethyldithiocarbamate"
J. Chromatogr. B 1994 Volume 652, Issue 2 Pages 203-210
Anita Andersson* and Hans Ehrsson

Abstract: Flash frozen plasma samples were thawed and ultrafiltered at 4000 g for 30 min using filters with a 10 000 mol. wt. cut off. The ultrafiltrate was stored at -25°C before use. Portions (20 or 50 µL) were analyzed on an anion-exchange 5 µm Nucleosil SB column (7 cm x 3.2 mm i.d. or 15 cm x 4.6 mm i.d.) for cisplatin and on a cation-exchange 5 µm Nucleosil SA column (15 cm x 3.2 mm i.d.) for the monohydrated complex, with 0.125 M succinic acid of pH 5.2/methanol (2:3) as mobile phase (0.5 ml/min). Post-column reaction was performed using a packed bed reactor steel column (50 cm x 0.35 cm i.d.) of glass beads (details given), operated at 115°C with a mobile phase (0.17 ml/min) of sodium diethyldithiocarbamate in aqueous methanol. Detection was at 344 nm. The RSD of the determination was 11.5% for 20 ng of the monohydrate and 8% for 9 ng of cisplatin.
Blood Plasma HPLC Glass beads Post-column derivatization

"Quantitative Determination Of Unchanged Cisplatin In Rat Kidney And Liver By High Performance Liquid Chromatography"
J. Chromatogr. B 1995 Volume 663, Issue 1 Pages 181-186
Kazuhiko Hanada, Naomi Nagai and Hiroyasu Ogata*

Abstract: A quantitative analytical method for measuring unchanged cisplatin (CDDP) and high- and low-molecular-mass metabolites (fixed and mobile metabolites) in rat kidney and liver was developed. Unchanged CDDP, separated from fixed and mobile metabolites in tissue homogenates by consecutive procedures of fractionation and ultrafiltration, was determined by high performance liquid chromatography (HPLC) with post-column derivatization. Although unchanged CDDP was found to be partly metabolized to fixed metabolites during the preparation of cytosolic ultrafiltrates, the recovery of unchanged CDDP gave a constant value (about 70%), which was independent of tissue type and CDDP concentration (from 1 to 10 µg/ml). The detection limit for unchanged CDDP in the cytosolic ultrafiltrate was 20 ng/ml, corresponding to a concentration detection limit of 65 ng Pt per g of tissue in the kidney and liver. The concentrations of fixed and mobile metabolites were determined as platinum concentrations in the tissue homogenate and in the cytosolic ultrafiltrate using atomic absorption spectrometry after correcting for transformation of unchanged CDDP to fixed metabolites. The distribution of unchanged CDDP, mobile metabolites and fixed metabolites in rat kidney and liver, after bolus injection of CDDP (5 mg/kg), was determined using this method.
Kidney Liver HPLC Post-column derivatization

"Validation Of A Liquid Chromatography Post-column Derivatization Assay For The Determination Of Cisplatin In Plasma"
J. Pharm. Biomed. Anal. 1994 Volume 12, Issue 2 Pages 265-271
H. H. Farrish*, P. -H. Hsyu, J. F. Pritchard, K. R. Brouwer and J. Jarrett

Abstract: Plasma (400 µL) was mixed with acetonitrile (400 µL), centrifuged and portions (200 µL) of the supernatant were mixed with 100 µL of 0.01 M citric acid/0.1 mM hexadecyltrimethylammonium bromide (CTAB) buffer (buffer A) of pH ~2.5 and 700 µL of CH2Cl2. After centrifugation, portions (150 µL) of the supernatant were analyzed by HPLC on a Shandon BDS-Hypersil C18 column (10 cm x 4.6 mm i.d.; 5 µm) coated with CTAB, operated at 25°C with a Brownlee Polymer RP guard column (1.5 cm x 3.2 mm i.d.; 7 µm), buffer A of pH 5 as mobile phase (0.7 ml/min) and post-column reaction (reagent flow rate 0.2 ml/min) with 0.117 mM potassium dichromate in an OPA reactor (0.2 ml), then at 30°C with 28.16 mM NaHSO3 in a CRX-390 reactor (1 ml) for detection at 290 nm. The calibration graph was linear from 0.06-30 µg/ml of cisplatin (I) and the inter-run RSD were 4-8.2% (n = 8); the recoveries of I from plasma and plasma/acetonitrile (1:1) supernatant were 105.6% and 103%, respectively. After 18 h at 4°C, 17% of I was lost in the plasma extract. The method was applied to drug interaction studies in dogs.
Blood Plasma HPLC Spectrophotometry Post-column derivatization

"A Sensitive Post-column Derivatization/UV Detection System For HPLC Determination Of Antitumour Divalent And Quadrivalent Platinum Complexes"
Chem. Pharm. Bull. 1995 Volume 43, Issue 1 Pages 108-114
Kizu, R.;Yamamoto, T.;Yokoyama, T.;Tanaka, M.;Miyazaki, M.

Abstract: Methods for the determination of cisplatin (I), oxoplatin (II), carboplatin (III), oxaliplatin (IV) and tetraplatin (IV), in human plasma ultrafiltrate and urine (100 l samples) are described). For I, an MCI gel CDR10 column (8 cm x 4.6 mm i.d.) was used with 10 mM acetate buffer of pH 5.5 containing 0.1 M Na2SO4 and 30% acetonitrile as mobile phase. For II a MCI gel CPK08 column of similar dimensions was used with 50 mM K2SO4 as mobile phase. For III, IV and V and Inertsil ODS-2 column (25 cm x 4.6 mm i.d.) was used with 10 mM acetate buffer of pH 5.5 containing 5% acetonitrile as mobile phase. The flow rate was 1 ml/min and the columns were operated at 40°C. Post-column reaction at 60°C and 0.3 ml/min was performed with 40 mM NaHSO3 in 10 mM acetate buffer of pH 5.5 in a reaction coil (10 m x 0.5 mm i.d.) for detection at 290 nm. The calibration graphs were linear for 0.05-20 M-I, 0.1-20 M II, and 0.2-20 M III, -IV and -V and the corresponding detection limits were 20, 40, 60, 100 and 60 nM; the intra-day RSD were <5%. The recoveries were >>95%. The method was used to study the pharmacokinetics of I and II in rabbits.
Blood Plasma Urine HPLC Spectrophotometry Post-column derivatization