University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Cellobiose

  • IUPAC Name: (2R,3S,4S,5R,6S)-2-(hydroxymethyl)-6-[(2R,3S,4R,5R,6R)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxane-3,4,5-triol
  • Molecular Formula: C12H22O11
  • CAS Registry Number: 528-50-7
  • InChI: InChI=1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2
  • InChI Key: GUBGYTABKSRVRQ-QRZGKKJRSA-N

@ ChemSpider@ NIST@ PubChem

Citations 3

"Characterization Of An Immobilized Hexose Oxidase Reactor For Mono- And Oligosaccharide Determination By Liquid Chromatography"
Anal. Chim. Acta 1993 Volume 284, Issue 2 Pages 281-290
P. C. Maes and L. J. Nagels*

Abstract: Hexose oxidase was extracted from the seaweed Chondrus crispus and immobilized onto aminopropylglass beads (50 nm pore size, 200-400 mesh) with glutaraldehyde (details given) which were then packed into a stainless-steel column (50 x 2.1 mm i.d.). This enzyme reactor was used in a flow injection analysis system for the LC determination of carbohydrates on a Bio-Rad Aminex HPX 87P column operated at 85°C with water as mobile phase (0.4 ml/min). Samples eluting from the column were mixed with a stream (0.1 ml/min) of 0.2 M sodium phosphate and carried to the enzyme reactor for production of H2O2 which was detected amperometrically with a large-volume wall-jet electrochemical cell equipped with a 3 mm diameter Pt-disc working electrode operated at +750 mV vs. SCE. The detection limits were 2 ng for glucose and galactose, 40 ng for maltose, cellobiose and lactose, 500 ng for mannose and 800 ng for xylose. The conversion efficiencies for 12 mono- and oligosaccharides were measured at different flow-rates. These efficiencies and the reactor selectivity were compared to those of glucose oxidase reactors.
Electrode LC Immobilized enzyme Glass beads Biorad

"A Combined Cellobiose Oxidase/glucose Oxidase Biosensor For HPLC Determination Online Of Glucose And Soluble Cellodextrines"
Anal. Biochem. 1993 Volume 214, Issue 2 Pages 389-396
Nordling M., Elmgren M., Stahlberg J., Pettersson G. and Lindquist S. E.

Abstract: Highly specific biosensors can be prepared by immobilizing flavin-containing oxidases in a redox polymer on an electrode surface. By combining one glucose oxidase and one cellobiose oxidase electrode in a flow cell we have made a sensor for flow injection analysis, or post-column quantification, of glucose, cellobiose, and higher cellodextrines in an HPLC system. Samples of different concentrations of glucose and cellobiose, separately or mixed, were injected into the mobile phase and the current response was recorded simultaneously from both electrodes. The recorded response peak heights could be used for calibration curves. The usable measuring ranges were roughly 50 µM - 50 mM for glucose and 5 µM - 80 mM for cellobiose. Soluble cellodextrines, Glc1-6, could be separated on a C18 column by isocratic elution and detected by the sensor.
HPLC Sensor Electrode Apparatus Detector

"Potentiometric Flow Injection Analysis Of Disaccharides Using The Hexacyanoferrate(III)-hexacyanoferrate(II) Potential Buffer Solution"
Bunseki Kagaku 1986 Volume 35, Issue 9 Pages 807-813
Ohura, H.;Imato, T.;Asano, Y.;Yamasaki, S.;Ishibashi, N.

Abstract: The sample solution is injected (140 µL) into a water carrier stream that merges with a stream (both 0.45 mL min-1) of 0.1 mM Fe(CN)63- - 0.1 mM Fe(CN)64- in 0.6 M NaOH. The mixture flows through a reaction coil (20 m x 0.5 mm) at 85°C and a cooling coil (6 m x 0.5 mm) at 25°C, and the potential of a redox electrode is measured. The peak height varies rectilinearly for 1 µM to 1.5 mM disaccharide, and the limit of detection is 0.25 µM. The sensitivities for lactose, cellobiose and melibiose relative to that for maltose are 1.09, 1.05 and 0.82, respectively.
Potentiometry Electrode Heated reaction