Abstract: Liposomes, containing entrapped water-soluble molecules, were tested in continuous-flow systems as a means of signal enhancement of the entrapped analyte. Liposomes containing carboxyfluorescein were detected fluorimetrically at 515 nm (excitation at 450 nm). Peak profiles were obtained for the system with use of a 187 µL sample loop, a packed bead column, a knitted delay tube and a straight open-bore tube; the flow rate was 0.5 mL min-1. The effect of these parameters on the peak profiles was discussed.