University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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4-aminobutyric acid

  • IUPAC Name: 4-aminobutanoic acid
  • Molecular Formula: C4H9NO2
  • CAS Registry Number: 56-12-2
  • InChI: InChI=1S/C4H9NO2/c5-3-1-2-4(6)7/h1-3,5H2,(H,6,7)
  • InChI Key: BTCSSZJGUNDROE-UHFFFAOYSA-N

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Citations 3

"Enzymic Flow Injection Determination Of γ-aminobutyric Acid"
Anal. Lett. 1995 Volume 28, Issue 2 Pages 259-266
Horie, H.;Rechnitz, G.A.

Abstract: Samples (20 µL) were mixed with 20 µL 2.5 µM-NADP/10 µM-2-oxoglutarate and injected into a carrier stream (0.1 or 0.8 ml/min) of 50 mM Tris hydrochloride buffer of pH 8 containing 1 mM 2-mercaptoethanol and 200 mM Na2SO4 which passed to an enzyme reactor. The reactor comprised a column (3.5 cm x 3 mm i.d.) packed with 200 mg glutaraldehyde-treated glass beads that had been mixed with 80 mg/ml GABAse (4-aminobutyrate:2-oxoglutarate aminotransferase and succinic semialdehyde:NADP+ oxidoreductase) in 0.2 mL 50 mM sodium phosphate buffer of pH 7 overnight, then washed with phosphate buffer. The NADPH produced was determined fluorimetrically at 460 nm (excitation at 360 nm). The calibration graphs were linear from 1-500 and 0.5-500 µM-γ-aminobutyric acid (GABA) at 0.8 and 0.1 ml/min, respectively. No RSD are given. At 0.8 ml/min samples could be injected every minute. The enzyme reactor retained >60% activity over 10 days. Recoveries of GABA from spiked green tea infusions were 97-104% at a flow rate of 0.1 ml/min.
Plant Fluorescence Glass beads Immobilized enzyme Column

"Fluorimetric Determination Of Secondary Amines By High Performance Liquid Chromatography With Post-column Derivatization"
J. Chromatogr. A 1983 Volume 264, Issue 3 Pages 423-433
Akira Himuro, Hiroshi Nakamura and Zenzo Tamura

Abstract: Amines were separated on columns (25 cm x 4.6 mm or 30 cm x 4.0 mm) of, respectively, Partisil-10 SCX or TSK LS-410; the respective mobile phase (pH 3) was 0.1 M citrate or ~50 mM phosphate buffer. The eluate was mixed with NaClO solution to convert secondary into primary amines; the reaction also decreased the detectability of primary amines with phthalaldehyde - 2-mercaptoethanol reagent. The products of reaction with the cited reagent (after addition of 2,2'-thiodiethanol to reduce excess of NaClO) were determined by fluorimetry at >405 nm (excitation at 360 nm). The optimum pH value and NaClO concentration. were: for prolines, ~8 and 0.03 mM, ~12 and 0.07 mM for adrenaline and 3-O-methyladrenaline, and ~14 and 0.15 mM for N-methylamino-acids (e.g., sarcosine). In an example, catecholamines and their 3-O-methyl derivatives were efficiently separated on the TSK column in ~40 min. Typically, the coefficient of variation for 1 nmol injected (n = 10) was 2.7% for proline and 1.7% for 4-aminobutyric acid.
HPLC Fluorescence Post-column derivatization

"New Continuous-flow-monitoring System For Radioactive Amino-acids"
Agric. Biol. Chem. 1981 Volume 45, Issue 8 Pages 1881-1884
Satoshi MORI

Abstract: A continuous flow system consisting of a high-speed amino acid analyzer and a radioliq. analyzer is described for determining radiolabeled amino acids. The flow cell of the radio-liq. analyzer is packed with Li glass scintillator that fluoresces upon b-irradn. The amino acid analyzer was a column (4.5 x 300 mm) packed with Durrum DC 4A resin, with Li citrate buffer as eluant, and ninhydrin as detection reagent at 570 or 440 nm. The system was tested on the assimilation of proline-U-14C in barley roots. The system had a counting efficiency for 14C of ~45% and a detection limit for 14C of 0.25 nCi. In barley roots, the order of specific activity after 60 min was proline > glutamic acid > glutamine > GABA > aspartic acid > asparagine. After 120 and 180 min, the order was proline > aspartic acid > glutamic acid > glutamine > GABA > asparagine.
Barley Radiochemical