University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Bacteria, salmonella

  • CAS Registry Number: NA

@ ChemSpider@ NIST@ PubChem

Citations 3

"Piezoelectric Flow Injection Analysis Biosensor For The Detection Of Salmonella Typhimurium"
J. Food Sci. 2002 Volume 67, Issue 1 Pages 314-320
Babacan S, Pivarnik P, Letcher S, Rand A

Abstract: Piezoelectric biosensors have the potential to provide direct detection of food contaminants, such as pathogens. In this study, Protein A antibody immobilization was used for the activation of the piezoelectric biosensor to detect Salmonella typhimurium. The overall system consisted of a new design for a flow cell and flow injection analysis system. The flow cell made possible a baseline stability of±1 Hz out of 5 MHz for hours. The sensor had responses of 5 to 65 Hz in 30 min with R-2 = 0.95 for S. typhimurium concentrations of 10(7) to 10(9) CFU/ml under continuous flow, and 3 to 75 Hz in 40 min with R-2 = 0.96 for S. typhimurium concentrations of 106 to 1010 CFU/ml under stop flow. Cross-reactivity tests were performed with nonpathogenic Escherichia coli, Escherichia coli O157.H7, Listeria monocytogenes, and Vibrio parahaemolyticus and showed less than 10% response.
Sensor Piezoelectric crystal Microbalance Interferences

"Highly Sensitive Flow Injection Immunoassay System For Rapid Detection Of Bacteria"
Anal. Chim. Acta 1999 Volume 399, Issue 1-2 Pages 99-108
Ihab Abdel-Hamid, Dmitri Ivnitski, Plamen Atanasov and Ebtisam Wilkins

Abstract: A flow injection amperometric immunofiltration assay system for the rapid detection of total Escherichia coli and Salmonella was developed. The system is based on the use of disposable porous nylon membranes which act as a support for the immobilization of anti-E. coli or anti-Salmonella antibodies. The assay system consists of a flow injection system, a disposable filter-membrane and an amperometric sensor. Parameters affecting the performance of the immunofiltration assay system such as membrane pore size, non-specific binding, conjugate concentration and sample volume were studied and optimized. A sandwich scheme of immunoassay was employed and the immunofiltration system was able to specifically and directly detect 50 cells/ml of total E. coli or 50 cells/ml of Salmonella with an overall analysis time of 35 min. This immunosensor can be easily adapted for the assay of other microorganisms and may be a basis for a new class of highly sensitive and automated bioanalytical devices for the rapid quantitative detection of bacteria.
Amperometry Sensor Membrane Optimization

"Piezoelectric Biosensor For Detection Of Salmonella-typhimurium"
J. Food Sci. 1997 Volume 62, Issue 5 Pages 1067-1071
Ye J, Letcher SV, Rand AG

Abstract: A flow injection analysis (FIA) system was developed based on a piezoelectric biosensor for detection of Salmonella typhimurium. The anti-Salmonella spp antibody was immobilized onto the gold electrode coated quartz crystal surface through a polyethylenimine-glutaraldehyde (PEG) technique and dithiobis-succinimidyl propionate (DSP) coupling. The PEG technique proved more successful for FIA applications than the DSP coupling. The biosensor had responses of 23-47 Hz in 25 min when the PEG immobilization technique was employed, with R-2>0.94 for Salmonella typhimurium concentrations of 5.3 x 10(5) to 1.2 x 10(9) CFU/mL. 17 References
Food Piezoelectric crystal Sensor Sensor Electrode Electrode