Abstract: The FIA system for the cited online monitoring systems are both shown schematically. Cellulose hydrosylate was diluted with phosphate buffer of pH 5.5 (used throughout) and passed through the β-glucosidase cartridge (at 50°C) at 0.27 ml/min. The converted sample was injected into a phosphate buffer stream (2 ml/min) and passed through the GOD cartridge (at 30°C) to the detector. The total sugar content was calculated using the glucose-based calibration graph. A second portion of diluted hydrolysate was passed through the GOD cartridge to determine glucose. For amygdalin (toxic waste product), the effluent was diluted with phosphate buffer and injected into the carrier stream (1.7 ml/min) and passed through both enzyme columns (at 30°C) to the detector. A second portion of the diluted effluent was injected in to the carrier stream and passed through the GOD cartridge. For each analysis, the concentrations of glucose were calculated using the glucose calibration graph and amygdalin was calculated by difference.

Abstract: A powdered sample (5 g) of bitter apricot seed was placed in a color comparison tube, then mixed and diluted to 50 mL with water. The tube was covered with a cover and heated at 37°C for 2 h on a water bath. A 50 mL portion of the supernatant solution was mixed with 10 mL of 1 mM NaOH and diluted with water to 100 mL. A 50 µL portion of the sample solution was injected into the carrier stream of NaOH of pH 10 (6 ml/min) and reacted on a CuS column to form Cu(CN)43-, which was determined by AAS at 324.7 nm. The calibration graph was linear from 1-100 µg/ml of cyanide and the RSD (n = 10) were 3%. Recoveries of amygdalin added to two different samples were 48% and 104%. Tolerance levels are given for eight anions.