University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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δ-Aminolevulinic acid

  • IUPAC Name: 5-amino-4-oxopentanoic acid
  • Molecular Formula: C5H9NO3
  • CAS Registry Number: 106-60-5
  • InChI: InChI=1S/C5H9NO3/c6-3-4(7)1-2-5(8)9/h1-3,6H2,(H,8,9)
  • InChI Key: ZGXJTSGNIOSYLO-UHFFFAOYSA-N

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Citations 3

"Fluorimetric Determination Of Urinary δ-aminolaevulic Acid By High Performance Liquid Chromatography And Post-column Derivatization"
J. Chromatogr. B 1988 Volume 426, Issue 1 Pages 365-369
Akira Okayama

Abstract: Urine was mixed with 10% trichloroacetic acid, and the solution was centrifuged. The supernatant solution was subjected to HPLC on a column (15 cm x 6 mm) of TSK-GEL SCX (5 µm) with stepwise gradient elution (flow rate 1 mL min-1) with 0.01 M NaH2PO4 buffer (pH 3) and 0.01 M Na2HPO4 - 0.05 M NaOH (program illustrated). The eluate was mixed, at 95°C, with 18.5% formaldehyde solution in 2 M acetic acid and then with water - ethanol - acetylacetone (1:1:2) and the fluorescence was measured at 473 nm (excitation at 363 nm). The calibration graph was rectilinear for 0.25 to 50 mg L-1 of 5-aminolaevulic acid, and the limit of detection was 100 pg. The within- and between-day coefficient of variation at the 200 ng level were 1.2 and 2.5%, respectively. The mean recovery was 100.2%.
Urine HPLC Fluorescence Heated reaction Post-column derivatization

"Determination Of δ-aminolevulinic Acid In Blood Using Stopped-flow HPLC"
Bunseki Kagaku 1994 Volume 43, Issue 4 Pages 311-316
Hosoda, K.;Omae, K.;Takebayashi, T.;Wada, H.;Sakurai, H.;Shibata, T.

Abstract: Pre-treated blood diluted with 100 mM sodium acetate of pH 5 was introduced into a condensing coil at 98°C and mixed with 50% acetylacetone in 25% ethanol followed by 10% formaldehyde solution (schematic diagram given). Analysis was by HPLC on a column (15 cm x 4.6 mm i.d.) of ODS-80ts (5 µm), with 1% acetic acid/methanol (47:53) as mobile phase (0.9 ml/min) and fluorescence detection at 463 nm (excitation at 373 nm). The detection limit was 2 µg/l of δ-aminolevulinic acid, which is a 2.5-fold improvement on the conventional method. RSD (n = 10) calculated from 4-time determinations/sample per week were 5%. The method was applied to 35 Pb-exposed and non-exposed workers and the results agreed well with those obtained by conventional HPLC. The method takes 15 min/sample and may prevent exposure to hazardous chemicals such as formaldehyde and acetylacetone.
Blood HPLC Stopped-flow

"Direct Injection Method For Quantitation Of δ-aminolevulinic Acid In Urine By High Performance Liquid Chromatography"
Chem. Pharm. Bull. 1992 Volume 40, Issue 7 Pages 1948-1950
Kondo M, Kimura H, Maekubo T, Tomita T, Senda M, Urata G, Kajiwara M

Abstract: For the cited determination, urine was directly injected onto a JASCO AA-pak Li HPLC column (10 cm x 6 mm) packed with cation-exchange resin (5 µm). HPLC was performed at 65°C with 0.1 M Li citrate (pH 4.9) as mobile phase (0.6 mL min-1) and fluorimetric detection at 440 nm (excitation at 350 nm) after post-column derivatization with o-phthalaldehyde. Calibration was rectilinear up to 60 nmol (with peak areas measured) and the detection limit was 10 pmol. The coefficient of variation was 2.65% and the recovery was 103%. Good correlation with a colorimetric method was observed (r = 0.979). The method was applied to the measurement of I in healthy volunteers, patients with acute intermittent porphyria and workers occupationally exposed to lead.
Urine HPLC Fluorescence Post-column derivatization