University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Amino acids, dansylated

@ ChemSpider@ NIST@ PubChem

Citations 4

"Signal Enhancement Of Dansyl Amino Acids By Fluorimetric Detection With A Packed Flow Cell In Microcolumn Liquid Chromatography"
Anal. Chim. Acta 1995 Volume 311, Issue 2 Pages 231-236
Toyohide Takeuchi* and Tomoo Miwa

Abstract: Signal enhancement of dansyl amino acids has been achieved by fluorimetric detection with a packed flow cell in microcolumn liquid chromatography. The same materials as in the separation column were packed in a 0.32 mm i.d. cylindrical flow cell, where the analytes were detected in the presence of the stationary phase. The present detection system greatly improved the sensitivity of the late-eluting analytes, compared with common fluorimetric detection with an empty flow cell. Both peak height and peak area of analytes increased with increasing retention time owing to the focusing and environmental effects of the stationary phase. The detection limit of the analyte eluting in ~70 min was improved by a factor of 73 in comparison with that achieved by common fluorimetric detection with an empty flow cell.
Fluorescence HPLC Post-column derivatization Reactor Flowcell Signal enhancement

"Flow Injection Analysis Using Continuous Channel Electrophoresis"
Anal. Chem. 1996 Volume 68, Issue 19 Pages 3441-3449
J. M. Mesaros, P. F. Gavin, and A. G. Ewing

Abstract: Continuous-zone electrophoretic separation performed with narrow channels (4.8 or 4.9 cm x 48 µm internal height) coupled to fused-silica capillaries (60 cm x 40 µm i.d.) were used to monitor dynamic chemical changes which occurred in a FIA system (described) and the fundamental aspects of the data generated are discussed in a comparison of static and dynamic analyzes. Data deconvolution was illustrated with the analysis of a dynamic separation and realistic analytical situations were simulated with three classes of dynamic analyzes. A mixture of α-dansylated amino-acids (listed) in 25 mM 3-(cyclohexylamino)-1-propanesulfonic acid of pH 9.6 was added to a FIA system (described) to provide a number of sample duration periods. A second procedure involved the staggered addition of short-duration plugs of analyte and α-dansyl-L-arginine (I) added continuously at different concentrations. Sample duration and analyte contact time with the capillary were determined with an error of M-I and concentration changes for 0.38-3.04 mM I were demonstrated with transitional edges on the time scale of 3-4 s.
Electrophoresis Deconvolution

"Application Of Bis-[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl] Oxalate To Post-column Chemiluminescence Detection In High Performance Liquid Chromatography"
J. Chromatogr. A 1987 Volume 400, Issue 1 Pages 169-176
Kazuhiro Imai, Yoriko Matsunaga, Yukie Tsukamoto and Atsuhiko Nishitani

Abstract: The sample was subjected to HPLC, at 30°C, on a column (15 cm x 4.6 mm) of TSK LS 120-T with a mobile phase (0.6 mL min-1) of 0.1 M imidazole nitrate (pH 7.0) - acetonitrile (7:3) and post-column derivatization with 0.05 mM bis-[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl] oxalate - 5 mM H2O2 in acetonitrile - ethyl acetate (1:1) and chemiluminescence detection. The method was applied in the determination of dansylated amino-acids.
Chemiluminescence HPLC Heated reaction Post-column derivatization

"Fluorimetric Detection Of Dansyl Amino-acids By Packed Flow Cell In Liquid Chromatography"
Chromatographia 1995 Volume 40, Issue 9-10 Pages 545-549
T. Takeuchi and T. Miwa

Abstract: Sample (21 µL) was analyzed on a 5 µm ODS L-column (15 cm x 4.6 mm i.d.) with 22% aqueous acetonitrile in 40 mM ammonium acetate of pH 7.2 as mobile phase (1 mL/min). Detection was performed with use of a packed flow cell (6 x 1.5 mm i.d.; 16 µL) packed with 5 µm L-column ODS packing slurried in methanol. Detection was at 528 and 522 nm for post-column and on-column detection, respectively (excitation at 355 nm). Calibration graphs were linear up to 40 µM dansyl amino-acid derivatives (tabulated). Some chromatograms are given. Detection limits were 0.12-1.4 µM and 0.04-0.21 µM for post-column and on-column detection, respectively. Detection limits for analytes eluting in around 1 h were improved by a factor of 28 compared with those achieved with a normal empty flow cell.
LC Fluorescence Post-column derivatization Flowcell