University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Alachlor

  • IUPAC Name: 2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide
  • Molecular Formula: C14H20ClNO2
  • CAS Registry Number: 15972-60-8
  • InChI: InChI=1S/C14H20ClNO2/c1-4-11-7-6-8-12(5-2)14(11)16(10-18-3)13(17)9-15/h6-8H,4-5,9-10H2,1-3H3
  • InChI Key: XCSGPAVHZFQHGE-UHFFFAOYSA-N

@ ChemSpider@ NIST@ PubChem

Citations 3

"Flow Injection Liposome Immunoanalysis (FILIA) For Alachlor"
Talanta 1994 Volume 41, Issue 10 Pages 1747-1753
Stuart G. Reeves*, Geoffrey S. Rule, Matthew A. Roberts, Alison J. Edwards and Richard A. Durst,

Abstract: A schematic diagram of the FILIA system is given. Samples of alachlor-tagged liposomes with a sulforhodamine B dye in TBS and alachlor in methanol were injected into a stream of TBS (200 µL/min) and onto a column (5 cm x 4.3 mm i.d.) packed with glass beads coated with anti-alachlor antibody (preparation details given). The column was washed for 5 min with 30 mM n-octyl-β-D-glucopyranosine to lyse the bound liposomes and release the fluorescent dye which was measured in a flow-through fluorimeter at 574 nm (excitation at 545 nm). The column was then washed with TBS for 5 min before the next sample injection. The effect of antibody concentration and flow rate on the sensitivity of the assay are discussed.
Biological Immunoassay Fluorescence Glass beads Liposomes

"Flow Injection Liposome Immunoanalysis (FILIA) With Electrochemical Detection"
Electroanalysis 1995 Volume 7, Issue 9 Pages 838-845
Alison J. Edwards *, Richard A. Durst

Abstract: Alachlor (I) in environmental samples was covalently bonded to dipalmitoyl phosphatidylethnolamine using N-succinimidyl-S-acetylthioacetate as described by Siebert et al. (Anal. Chim. Acta 1993, 282, 297). The I-bonded lipid was then dissolved in an organic solvent (not specified) and reacted with aqueous 100 mM potassium ferrocyanide of pH 7.4. After sonication and removal of the organic solvent, the liposome suspension was reacted with further potassium ferrocyanide (no details given) at 45°C, filtered, gel-filtered and dialysed. The dialysed liposomes were then diluted in PBS of pH 7.4 and 20 µL injected onto a column (5 cm x 4.3 mm i.d.) containing I-antibody-immobilized glass beads (preparation details given), PBS as mobile phase (0.55 ml/min) and amperometric detection at +330 mV vs. Ag/AgCl. Calibration graphs were linear for 1-10% liposomes bound on-column, with a detection limit of 0.5 ng I on-column.
Environmental Amperometry Glass beads Immobilized antibody Liposomes

"Use Of Protein A In A Liposome-enhanced Flow Injection Immunoassay"
Anal. Proc. 1994 Volume 31, Issue 11 Pages 339-340
Geoffrey S. Rule, Derek A. Palmer, Stuart G. Reeves and Richard A. Durst

Abstract: A glass column (10 cm x 6 mm i.d.) containing controlled-pore glass coated with protein A was used in the cited immunoassay for alachlor (I). The column was activated by injecting anti-I antibody (raised in rabbits) onto the protein A matrix. Alachlor (1 mg/ml in methanol) was diluted with TBS of pH 7.4 and injected onto the column. Liposomes (100 mM sulforhodamine B in TBS encapsulated in lumen) were then injected onto the column. A detergent solution (octyl β-L-glucopyranoside) was then passed through the column. The fluorescence generated by the released sulforhodamine B was measured at 572 nm (excitation at 556 nm). The antibody was then removed with 20% acetic acid (pH 2.2) and the column reconditioned for the next analysis by returning the mobile phase to pH 7.4 TBS. Assays were conducted at a flow rate of 0.8 ml/min. Each analysis took 11 min. The detection limit was 10 ng of I injected.
Biological Immunoassay Fluorescence Controlled pore glass Liposomes